摘要
合成了简称Ｃ１８的十八烷基－２－氨基－２－脱氧－β－Ｄ－吡喃葡萄糖甙．通过π－Ａ曲线研究了Ｃ１８和ＧＯＤ（葡萄糖氧化酶）的相互作用，并用光谱方法研究了转移的Ｃ１８／ＧＯＤ混合ＬＢ膜的光谱特性．从圆二色性（ＣＤ）光谱结果可以看出，固定在Ｃ１８膜里的ＧＯＤ二级结构发生了变化．低温荧光实验显示混合膜中一部分ＧＯＤ仍然保持其天然结构，但另一些则发生了部分变性．
おlkyl 2amino2deoxyβDglucopyranoside called C18 was synthesized. The interaction between C18 and GOD (glucose oxidase) was studied by recording isotherms of monolayers. The transferred mixed C18/GOD LB films were investigated by means of spectroscopy methods. From the results of CD (circular dichroism) spectrum, GOD immobilized in C18 films will partly change their secondary structure. According to the low temperature fluorescence experiment, part of GOD in the LB film keeps native structure, others of them become partly denatured.
出处
《感光科学与光化学》
CSCD
1998年第1期32-37,共6页
Photographic Science and Photochemistry
基金
国家自然科学基金
中国科学院重大基础研究
关键词
葡萄糖检测
传感器
葡萄糖氧化酶
光谱
LB膜
glucose oxidase
circular dichroism spectrum
fluorescence spectrum
LangmuirBlodgett(LB) film
