摘要
目的:建立快速筛查感染性腹泻标本的实时荧光定量PCR法,考察其在群体感染性腹泻病原体快速筛查中的应用。方法:采用国家标准的细菌培养法(或传统ELISA法)对2006年丽水地区发生的总计14起共487例群体感染性腹泻标本进行病原体筛查,同时采用实时荧光定量PCR方法对该487例标本进行检测,比较两者的实验结果,并考察实时荧光定量PCR方法的特异性和灵敏度。结果:实时荧光定量PCR检测结果与传统检测方法的符合率为97.54%,除完全符合的278例标本之外,另有7例采用传统方法检测为阴性的标本用实时荧光定量PCR检测显示为阳性,表明实时荧光定量PCR方法具有更高的灵敏度;特异性和灵敏度实验表明实时荧光定量PCR方法的特异性达100%,灵敏度为102拷贝/ml。结论:实时荧光定量PCR法具有准确性好、灵敏度高、检测快速等优点,是一种理想的群体感染性腹泻病原体快速筛查的方法。
Objective：To establish the method of real -time fluorescent quantitative PCR（qPCR） and to examine its utility for rapid pathogens＇ screening in population of infectious diarrhea. Methods：There were 487 specimens of 14 infectious diarrhea outbreak events occurred in Lishui during 2006, which were examined by culture method of National Standards （ or traditional ELISA method, for virus） and compared with the qPCR method. Results：The result of qPCR was 97. 54% as compared with traditional methods. There were 278 specimens detected to be positive by both traditional and qPCR methods. Besides, 7 traditional - method - negative specimens were detected to be positive by qPCR, suggesting a higher sensitivity. The absolute specificity and sensitivity of qPCR were 100% and 102 copies/ml, respectively. Conclusion：qPCR method is ideal for rapid pathogen screening in population of infectious diarrhea because of its high accuracy and sensitivity.
出处
《中国卫生检验杂志》
CAS
2008年第6期1000-1003,共4页
Chinese Journal of Health Laboratory Technology
关键词
实时荧光定量PCR
感染性腹泻
快速筛查
Real time fluorescent quantitative PCR： Infectious diarrhea
Rapid screening
