摘要
本研究克隆了浙东白鹅催乳素受体基因(PRLR)的部分序列,并应用荧光定量PCR技术研究了浙东白鹅在产蛋期、就巢期和恢复期时PRLR基因在下丘脑、垂体和卵巢中的表达特点,结果表明,浙东白鹅PRLR基因的表达量在产蛋期、就巢期和恢复期差异显著(P<0.05),就巢期表达量最高,产蛋期次之,恢复期表达量最低。对不同组织PRLR的表达量分析,垂体最多,其次是下丘脑,卵巢中的表达量最低,垂体与卵巢中的表达量、卵巢与下丘脑的表达量均有极显著的差异(P<0.01),垂体与下丘脑中的表达量差异不显著(P>0.05)。
This study was conducted to clone partial sequence of 1 305 nucleotides of the prolactin receptor （PRLR） gene in Eastern Zhejiang White geese from genome DNA. Meanwhile, the PRLR gene expression characteristics were investigated during different reproductive periods by real time PCR. Comparisons of concentration of prolactin receptor mRNA in the hypothalamus, pituitary gland and ovary of adult female geese at egg-laying, out-of-lay and incubating periods were made, respectively. The results indicated that there were significant difference （P〈0.05） in PRLR mRNA expression between different reproduction periods of the geese. The lowest level of PRLR expression was found in out-of-lay geese, higher in the egg-laying geese, and the greatest in incubating geese. Furthermore, the analysis of PRLR expression in different tissues indicated that the highest levels of PRLR was expressed in the pituitary gland, followed in hypothalamus, and the least in ovary of the geese. There were significant difference（P〈0.01）of PRLR expression between the pituitary gland/hypothalamus and ovary of the geese, whereas no any difference was observed between the pituitary gland and hypothalamus.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第6期823-826,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家科技部“973”项目(2006CB102100)
浙江省科技重大项目(2005C12005-2)
关键词
催乳素受体基因
浙东白鹅
繁殖时期
荧光定量PCR
prolactin receptor gene
Eastern Zhejiang White geese
reproduction periods
real time PCR
