摘要
[目的]对杂交兰原球茎的增殖及分化进行研究。[方法]采用L9(34)正交试验设计,研究培养基、6-BAI、BA和AC对杂交兰原球茎增殖的影响;设置几种不同培养基对原球茎的分化进行探讨。[结果]培养基和6-BA对原球茎的增殖影响不大,IBA浓度为0.1 mg/L、AC浓度为1.0 mg/L时,原球茎增殖效果最好;1/2 MS+6-BA 1.0 mg/L+NAA 0.5 mg/L对原球茎的分化效果最好。[结论]在培养基中添加NAA有利于原球茎的分化。
Abstract [ Objective ] The research aimed to study the propagation and differentiation of PLB of Cymbiditun hybridum × faberi . [ Method ] Four facts of medium,6-BA,1BA and AC with three different level L9（3^4） were designed to study the effects on propagation of PLB of Cymbidium hybridum × faberi. Scveral different mediums were used to study the differentiation of PLB of Cymbidium hybrduum × faberi. [ Results] Different concentration among the factors of 1BA and AC influenced the propagation of PLB directly. There was no difference with different kinds of mediums and different concentrations of 6-BA. 1BA 0.1 mg/L and AC 1.0 mg/L was favorable to the propagation of PLB. The best differentiation medium was 1/2 MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.5 mg/L. [ Conclusion] The medium with NAA was beneficial to the differentiation of PLB of Cymbidium hybridum × foberi.
出处
《安徽农业科学》
CAS
北大核心
2008年第13期5309-5310,5353,共3页
Journal of Anhui Agricultural Sciences
基金
郑州市科技攻关项目(051SGDS17017)
关键词
杂交兰
原球茎
增殖
分化
Cymbidium hybridmn × foberi
Protocorm like-body
Propagation
Differentiation
