摘要
以‘嘎拉’苹果(Malusdomestica Borkh.‘Gala’)叶片为试材,研究了幼叶、展开叶、成熟叶、衰老叶4个不同时期叶片的抗坏血酸(AsA)含量、合成和代谢相关酶活性及其相互之间的关系。结果表明,不同叶龄苹果叶片总抗坏血酸和AsA含量的变化趋势一致,均在成熟期最高,衰老期开始下降,AsA关键合成酶GalLDH活性表现出相应的变化。同时,有助于AsA再生的脱氢抗坏血酸还原酶(DHAR)、单脱氢抗坏血酸还原酶(MDHAR)、谷胱甘肽还原酶(GR)活性均在叶片衰老期最低,并伴有抗坏血酸过氧化物酶(APX)活性的下降和H2O2的大量积累,使抗坏血酸氧化程度在衰老期也达到最大。
Ascorbic acid （AsA） metabolism, including AsA content, metabolism relative enzymes activities and relationships among them, was investigated in leaves of apple （Malus domestica Borkh. ＇ Gala＇ ） of various ages, i.e. young leaves, expanding leaves, mature leaves, aged leaves. The results showed that total AsA content and AsA content aged leaves, and changes changed accordingly, reaching maximum in mature leaves and minimum in of GalLDH activity were accordant with AsA content. With the decline of APX activities and accumulation of H2O2, the activities of MDHAR, DHAR and GR which conduce to regeneration of AsA were lowest at aged leaves, so as to the extent of AsA oxidation reached maximum in aged leaves.
出处
《园艺学报》
CAS
CSCD
北大核心
2007年第4期995-998,共4页
Acta Horticulturae Sinica
基金
农业部‘948’项目(2006-G28)
西北农林科技大学"拔尖人才支持计划"项目(2005-2)
关键词
苹果
叶片
叶龄
抗坏血酸
Apple
Leaf
Leaf age
Ascorbic acid
