摘要
目的:观察以抑癌基因p53编码蛋白在正常肠黏膜、肠上皮化生及结直肠癌组织中的表达,探讨结直肠癌组织中p53和mRNA的表达及意义。方法:用半定量逆转录聚合酶链反应(RT-PCR)检测64例结直肠癌及其癌旁肠上皮化生组织中p53蛋白的表达水平,比较其与结直肠癌组织学分型、临床病理分期的关系。结果:1)结直肠癌组织中p53蛋白的表达明显低于正常组织(66.7%,26/39)。结直肠癌组织p53蛋白mRNA的表达水平与正常黏膜组织相比较,显著偏低(1.12±1.07,2.32±1.05,P<0.05)。2)蛋白和基因表达呈显著正相关(r=0.875,P<0.01)。3)黏液腺癌组为3/4,管状、乳头状腺癌为19/35,p53蛋白和基因表达与结直肠癌临床病理、组织学分型无明显相关性,P>0.05。结论:p53失活或低表达与结直肠癌的发生有密切相关性。提示p53 mRNA表达水平的检测可作为判定结直肠癌发生及侵袭转移能力的一项客观指标。
OBJECTIVE： To detect the expression of p53 protein mRNA in intestinal metaplasia （IM） and colorectal neoplasms,and investigate its clinical implication in tumorigenesis and progression of coloreetal neoplasms. METHODS： Formalin fixed paraffin embedded tissues from 64 cases of colorectal neoplasms and IM of their adjacent mucosa were evaluated for P53 protein expression by the immunohistochemistry method and RT-PCR, and the results were compared with cilincal parameters. RESULTS： The level of mRNA of P53 protein decreased more significantly in adenocarcinoma in normal mucous membrance tissue （1.124±1.07,2.32±1.05, P〈0.05）. No correlation existed between clinical pathology and histology sort,P〉0.05. CONCLUSION： Loss or reducing expression of p53 protein occurs commonly in colorectal neoplasms. It is suggested that p53 protein can be a useful marker for predicting invasion and metastatic ability of colorectal neoplasms.
出处
《中华肿瘤防治杂志》
CAS
2007年第8期607-609,共3页
Chinese Journal of Cancer Prevention and Treatment
关键词
结直肠肿瘤/病理学
蛋白质p53/分析
基因表达
逆转录聚合酶链反应
colorectal neoplasms/pathology
protein p53/analysis
expression of gcne reverse transciption-polymer-ase chain reaction
immunohistochemistry
