摘要
为探讨利用鳞翅目昆虫对环境激素进行生物学评价和监测的方法,用2,3-二苯基溴化四唑(MTT)、4’,6二乙酰基-2-苯基吲哚(DAPI)荧光染色和单细胞凝胶电泳(SCGE)、流色细胞仪检测(FCM)等技术,分析检测了环境激素阿特拉津(AT)对体外培养家蚕卵巢细胞(BmN)细胞增殖和凋亡的影响。结果显示：AT浓度超过0．0625mmol/L,显著抑制了BmN培养细胞48h的存活率(p＜0．001),LC_(50)为0．0703mmol/L；0．0625～0．5000mmol/L AT处理24h后,BmN细胞核出现明显的凋亡现象；0．5000mmol/L的AT处理48h后,BmN细胞DNA链发生断裂。AT对BmN细胞增殖和凋亡的影响有明显的时间-剂量效应。长期处于AT污染的环境中对鳞翅目昆虫可能有严重的细胞毒性。
In order to explore the biological methods by using lepidopterous insect to evaluate and monitor environmental hormone, the inhibitory effect of atrazine （AT） on the cell growth and apoptosis of ovary cells of Bombyx silkworm （BmN） in vitro was measured by MTT[3-（4,5）dimethylthiazol-2,5-diphenyl-tetrazolium bromide], SCGE （single-cell gel electrophoresis）, DAPI （4＇,6-diamidino-2-phenylindole） and FCM （flow cytometer） assays. Results showed when the concentration of AT is over 0.062 5 mmol/L, it inhibits BmN cell survival rate of 48 h remarkably （p 〈 0.001）, and the LC50 is 0.070 3 mmol/L. After 24 hours, the nucleolus of cells treated by 0.062 5～0.500 0 mmol/L AT appeared apoptosis clearly. AT can induce the breakage of DNA after 48 hours cultured with 0.500 0 mmol/L. AT has remarkable time-dosage effects on the cell growth and apoptosis. The results suggested that living in the condition polluted by AT for a long time, cells of lepidopterous insect be poisoned badly.
出处
《蚕业科学》
CAS
CSCD
北大核心
2006年第4期497-501,496,共6页
ACTA SERICOLOGICA SINICA
基金
国家重点基础研究发展计划"973项目"(编号2005CB121005)
苏州大学科研基金(编号Q3113403
Q3138539)。
