摘要
目的:观察肝硬化患者血流动力学变化情况及血浆内毒素(LPS)、肿瘤坏死因子-α(TNF-α)和NO的水平并探讨之间相关关系。方法:试验检测血浆内毒素,双抗体夹心ELISA检测TNF-α,NO2-/NO3-测定采用硝酸还原法,并对CO、MAP、SVRI行同步观察。结果:健康组与肝硬化患者比较,失代偿组高动力循环状态特征明显。失代偿组、代偿组、健康组LPS、TNF-α、NO依次明显升高(P<0．01)。血浆LPS与TNF-α和NO之间呈直线正相关。血浆NO水平与CO呈显著性正相关;与MAP、SVRI呈显著性负相关。结论:肝硬化患者血浆LPS、TNF-α、NO生成增多,NO在肝硬化血液动力学紊乱中起重要作用。
Objective： To study the hemodynamics state and the plasma levels of nitrous oxide （NO）, tumor necrosis factor-α（TNF-α） and Endotoxin （LPS） and their relationship, Methods： Cardiac output （CO）, systemic vascular resistance index （SVRI） and mean arterial pressure （MAP） were measured; plasma endotoxin was observed using the Limulus test; plasma TNF-α was detemlined by the ABC-enzyme-linked immunosorbent assay; the plasma level of NO was detected using nitric reductase, Results： The characteristic of hyperkinctic state of circulation was obvious in the decompensated cirrhotic patients. Plasma endotoxin, TNF-α and NO levels were higher in decompensated cirrhotic patients than those in compensated cirrhotic patients, which were the highest in normal controls. There are positive correlations among LPS, TNF-α and NO in cirrhotic patients, The level of NO had a positive correlation with CO and had a negative correlation with MAP and SVRI. Conclusions： Hyperkinetic circulation in cirrhosis is more obvious in those with more severe liver dysfunction, NO might play an important role in hemodynamics dysfunction.
出处
《现代生物医学进展》
CAS
2006年第11期67-69,共3页
Progress in Modern Biomedicine
