摘要
目的:探讨红花注射液预防动静脉内瘘术后血栓形成的机制。方法:24只家兔被随机分为红花组、肝素组和对照组,每组8只;各组动物分别行双下肢双侧股动静脉内瘘术;术后即刻红花组和肝素组分别经耳缘静脉注射红花注射液(5 m g/kg)和肝素注射液(6 U/kg),对照组给予等量生理盐水,以后每日给药1次,连用14 d。各组动物分别于术后1、3、7和15 d经耳缘静脉采血检测血浆内皮素(ET)和血清一氧化氮(NO)含量。结果:术后红花组和肝素组血浆ET含量均显著低于对照组,血清NO含量均显著高于对照组(P均<0.01),术后各时间点红花组与肝素组血浆ET含量比较差异均无显著性;术后3 d和7 d红花组血清NO含量均显著高于肝素组(P均<0.01),术后1 d和15 d两组比较差异均无显著性。结论:家兔股动静脉内瘘术后ET增多,NO减少,存在血管内皮功能失调;红花注射液通过调整术后ET和NO的平衡,可改善血管内皮功能,防止早期血栓形成。
Objective： To study the mechanism of safflower injection （红花注射液） on preventing thrombosis after arteriovenous fistula operation in rabbits. Method： Twenty-four rabbits were randomly divided into three groups： safflower injection group, heparin group and control group （each n= 8）. Rabbit femoral artery vein side-to-side anastomosis models were performed on both sides of the lower extremities and they were coincided. Safflower injection （5 mg/kg） or heparin injection （6 U/kg） was given from ear edge vein immediately after operation in the respective group, while normal saline （0.9%） was given with the same fashion in the control group, once per day for 14 days. On the 1 st, 3 rd, 7 th and 15 th day after operation, the nitric oxide （NO） in serum and endothelin （ET） in plasma were determined respectively. Results; The plasma ET contents were significantly lowered in safflower and heparin groups compared with that of control group （both P〈0.01）. There was no difference between safflower group and heparin group at each time point. The serum NO contents were significantly elevated in safflower and heparin groups compared with that of control group （both P〈0.01）. On the 3 rd and 7 th day after operation, the serum NO contents in the safflower group were higher than those in the heparin group （both P〈0.01）. There were no differences between safflower group and heparin group on the 1 st and 15 th day. Conclusion： The safflower injection has the effect of improving the endothelial function after femoral arteriovenous fistula operation in rabbits by the adjustment of plasma ET and serum NO. Safflower injection is worthy of clinical selection for anti-thrombosis.
出处
《中国中西医结合急救杂志》
CAS
2006年第6期345-347,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
关键词
红花注射液
动静脉瘘
内皮素
一氧化氮
safflower injection
arteriovenous fistula
endothelin
nitric oxide
