摘要
目的探讨血小板糖蛋白特异性抗体在再生障碍性贫血(AA)患者发病中的作用。方法采用改良的MAIPA法,检测血浆血小板糖蛋白特异性抗体(抗GPⅡb/Ⅲa抗体和抗GPIb/IX抗体)。若患者的OD值大于正常OD值的均数+3倍标准差则为阳性。结果AA组总阳性率为20.00%(6/30),特发性血小板减少性紫癜(ITP)组总阳性率为46.67%(14/30),正常对照组阳性率为0。总阳性率的比较:AA组与ITP组,P<0.05,两者之间有显著性差异。结论部分AA患者的糖蛋白特异性抗体为阳性,这表明部分AA患者的血小板减少与免疫因素有关,血小板糖蛋白特异性抗体所致的血小板破坏在AA患者的血小板减少中可能具有一定作用,这为AA的免疫抑制剂治疗提供了新的依据。
Objective To find the function of platelet specific antibodies against glycoproteins during the pathogenesis of aplastic anemia （AA） .Methods The plasma antibodies against GPⅡ b/Ⅲa and GPIh/IX were measured by using a modified monoelonal antibody specific immobolization platelet anfigens assay （MAIPA）. Absorbance greater than mean value plus treble standard deviation recorded for the normals were regaded as positive. Results In the patients with AA, the total positive rate was 20.00% （6/30）. In the patients with riP, tile total positive rate was 46.67 % （14/30）. There was no positive finding in the healthy control. Comparing the total positive rate： the difference between AA group and ITP group were significant（ P 〈 0.05）. Conclusion We found partial of the patients with AA and MDS had plasma specific antibodies against platelet GPⅡ b/Ⅲ a and GP Ⅰ b/IX by using a modified MAIPA assay. These results show that glycoprotein-specific antibodyy-mediated platelet destruction may contribute at least in part to AA. And the results also provide new basis for immunosuppression theranv using, in AA.
出处
《中国实验诊断学》
2006年第11期1269-1271,共3页
Chinese Journal of Laboratory Diagnosis
