摘要
目的探讨和评价新型农药—吡虫啉对农田生态系统中两栖动物的潜在风险,为其合理使用和科学管理提供基础数据。方法以青蛙为指示生物,应用急性毒性试验、微核试验和彗星试验来研究其急性毒性和遗传毒性。结果吡虫啉对蝌蚪急性毒性的LC50-48 h值为218.8 mg/L,表现为低毒;微核试验中,当其处理浓度高于8 mg/L时,能引起蝌蚪红细胞微核率的升高,与阴性对照组相比差异有显著性(P<0.05,P<0.01),并存在一定的剂量-效应关系(r2=0.96);在彗星试验中,所有吡虫啉处理组的彗星细胞率与阴性对照组相比差异有极显著性(P<0.01),细胞的DNA损伤表达成专用单位后与试验浓度存在明显的剂量-效应关系(r2=0.92)。结论该农药对青蛙的急性毒性为低毒,但微核试验和彗星试验都显示其对青蛙具一定的遗传毒性。
Objective To assess the risks of a novel pesticide, imidacloprid, on amphibians in the agricultural ecosvstern and provide the information for the better managelnents. Methods The Acute Toxicity Test, Micnmucleus Test and Comet Assay were used to determine the acute toxicity and/or genotoxicity of it on tadpoles and frogs of Rana nigronaculata Hallowell. Results The results showed that the value of LC50 - 48 h of it on the tadpoles was 218.8 mg/L. Compared with the negative controls, there were significantly increasing miemnueleus frequencies observed in the treated groups, in which the administrated concentration of imidacloprid was above 8 mg/L （P 〈 0.05, P 〈 0.01） , and close dose - effect relationship r^2 = 0.96） was observed in the Micronucleus Test. In the comet assay with frog＇s erythrocytes, there were very significant differences （P 〈 0.01） between the ＂comet cell＂ frequencies of all the tested groups and that of the negative controls. Conclusion The results of micronucleus test and comet assay suggested there are some genotoxicity risks of it to Rana nigronaculata Hallowell.
出处
《南华大学学报（医学版）》
2006年第2期181-184,共4页
Journal of Nanhua University(Medical Edition)
基金
有机地球化学国家重点实验室开放基金(OGL-200401)
南华大学启动基金(5-04-XJQ-04003)
衡阳市科技计划项目(2005KS01-001)
关键词
吡虫啉
黑斑蛙(青蛙)
急性毒性
微核
彗星试验
imidacloprid
tana nigronaculata hallowell
acute toxicity
micronuclei
comet assay
