摘要
目的观察经颅磁刺激(transcranialmagneticstimulation,TMS)对大鼠大脑中动脉栓塞后梗死周边区生长相关蛋白43(GAP43)和突触素(Syp)表达的影响。方法50只雄性SD大鼠应用线栓法建立大鼠大脑中动脉闭塞(MCAO)模型,并随机分为TMS组和自然恢复组,前者给予TMS治疗,后者常规饲养,另选5只雄性SD大鼠作为对照组。以免疫组化及图像分析技术检测对照组及两缺血组缺血后1、3、7、14、28d梗死周边区GAP43和Syp的表达。结果脑缺血后梗死周边区GAP43表达自术后1、3d开始增高,第7d达到高峰,自第14d开始降低;Syp于缺血后1d表达减少,3d达最低值,7d开始增高但不及对照组,14、28d表达高于对照组;TMS组梗死周边区GAP43和Syp的表达分别在7、14d和14、28d时高于自然恢复组。结论TMS可促进脑卒中后梗死周边区GAP43和Syp的表达,并可能具有促进突触再建和增强、完善再建突触效能的作用。
Objective To study the effects of TMS on the expressions of GAP-43 and Syp in rats after focal cerebral infarction, so as to explore the material foundation for TMS in improving neurorehabilitation. Methods 50 male SD rats were used to focal cerebral infarction model of middle cerebral artery occlusion （MCAO） by intraluminal filament occlusion and then divided into model group and TMS group. The TMS group was treated with TMS. And another 5 male SD rats were put into normal group without operation and treatment. The expressions of GAP-43 and Syp were detected ld, 3d, 7d, 14d and 28d after MCAO with immunohistochemistry. Results Compared with normal group, GAP-43 immunoreactivity incerased at 24 hours after MCAO in the boundary zone of the infarcts in model group and TMS group, and reached the peak on the 7th day, and declined in the later days, but it still remained high level on 28^th day. The expression of Syn downregulated remarkably in the boundary zone of the infarcts in model group and TMS group on 1^st day after MCAO, reached its lowest level at 3d, and slightly increased at 7d. Compared with normal group. Syp immunoreactivity increased on 14d and 28d after MCAO in the boundary zone of the infarcts in model group and TMS group. The expression of GAP-43 and Syp was remarkably higher in TMS group than model group on 7d, 14d and 14d, 28d respectively. Conclusions TMS could upregulate the experssions of GAP-43 and Syp in the boundary zone of the infarcts in rats with cerebral infarction.
出处
《卒中与神经疾病》
2006年第1期15-18,共4页
Stroke and Nervous Diseases
关键词
脑缺血
经颅磁刺激
生长相关蛋白-43
突触素
大鼠
Cerebral ischemia Transcranial magnetic stimulation（TMS） Growth associated protein-43 Synaptophysin Rats
