摘要
目的:以多药抗药细胞系k 5 6 2 /Adr为对象,研究参麦注射液无细胞毒性的剂量,能否逆转该细胞对阿霉素(Adr)及长春新碱(Vcr)的抗药性。方法:MTT法验证细胞的多药抗药性(MDR) ;MTT法计算药物对细胞的毒性作用;选用无毒性作用的三个剂量及两个药物与细胞作用的时间点,研究是否具有逆转作用及其与剂量和时间的关系。用流式细胞术检测细胞膜表面p -糖蛋白的表达变化。结果:k 5 6 2 /Adr细胞对阿霉素及长春新碱的抗药倍数分别为14 .37和70 7.88;对细胞的毒性历时4 8小时及72小时无明显变化(P >0 .0 5 ) ;选用4 8小时及72小时两个时间点,分别以0 .2mg/ml、0 .5mg/ml及1mg/ml三个无毒剂量试验,发现注入参麦注射液(SM)的k 5 6 2 /Adr细胞对阿霉素的抗药性具有部分的逆转效果,并且具有一定的剂量依赖性,未见明显的时间依赖性。而对Vcr的抗药性未见明显的逆转效果。其逆转机理与部分下调细胞膜表面p -糖蛋白的高表达有关。
Objective:To explore the reversal of multidrug resistance by shenmai injection in leukemia multidrug resistance cell line,We chose the dosage that is safe to it.Methods:After confirming the multidrug resistance by MTT assay,We observed shenmai injection for its virulence to the cell lines.We chose three dosages that are safe to the cell and three points of time to explore how it works.The expression of p-gp in membrane was studied by flow cytometric analysis.Result:the adriamycin and vincristine are 14.37 and 707.88.during 48 hours or 72 hours,The virulence to the cell are almost the same (P>0.05).We chose two points of time,48 and 72 hours,Three dosages of 0.2 mg/ml,0.5 mg/ml and 1 mg/ml.We found that although shenmai injection can reserve adriamycin resistance to some degree which depends on the dosage,but can't reserve vincristine resistance.The mechanism was thought to decrease the expression of p-gp to some degree.
出处
《大同医学专科学校学报》
2005年第2期3-5,共3页
Journal of Datong Medical College
