摘要
目的 研究 17-β雌二醇对血管性痴呆 (vasculardementia ,VD)大鼠脑组织脑源性神经营养因子 (BNDF)表达的影响。方法 采用结扎双侧颈总动脉方法制备慢性前脑缺血动物模型 ,应用Y迷宫 ,免疫组化及ELISA法等检测腹腔注射 17-β雌二醇60d后VD大鼠认知功能以及脑组织中BDNF含量变化。结果 与正常对照组对比 ,大鼠学习尝试次数 ,记忆测试 10次的正确次数在造模 60d后差异有显著性意义 (P <0 0 1) ,腹腔注射 17-β雌二醇 60d后 ,大鼠认知功能显著改善 ;造模 60d后的大鼠 ,其脑内BD NF免疫阳性细胞数较正常对照组显著增高 ,雌二醇组大鼠脑组织内BDNF免疫阳性细胞数较模型组显著增高。BDNF在三组大鼠脑内含量的测定结果与之一致。结论 腹腔注射 17-β雌二醇可显著改善VD大鼠的认知功能 ,增加VD大鼠脑内BDNF含量。
Objevtive To observe the effects of intra-abdomina infusion of 17β-estrogen on the expressions of brain-derived neurotrophic factor(BDNF) in vascular dementia(VD) rats. Methods Forty-eight male Wister rats were randomly divided into three groups, that is ,control group (n=16). Model group(n=16) and test group (n=16). Model rats of chronic forebrain ischemia were subjected to permanent bilateral common carotid arteries ligation for 2 months.The rats of three groups were intra-abdomina infused with oil,oil and 17β-estrogen respectively. Cognitive function and the contents of BDNF were observed at 60d after injection of oil or 17β-estrogen by Y maze test, immunohistochemistry and enzyme-linked immnoadsorbent assay(ELISA). Results The number of learing , the correct times for 10 maze tests of VD rats at 60d after ligation were significantly different from those of the normal rats (p<0.01). significant recovery of cognitive impairment was found in VD rats infused with 17β-estrogen after 60d . The expressions of BDNF of the VD rats were significantly increased at 60d after ligation,compared with the control group. The expressions of BDNF in the test group were significantly higher than those of the model group .So were the contents of BDNF in three groups. Conclusion Intra-abdomin administration of 17β-estrogen significantly improves the cognitive function and increases the contents of BDNF in VD rats.
