摘要
采用非连续性缓冲系统聚丙烯酰胺凝胶电泳法,分别对50头九龙牦牛和50头麦洼牦牛的血清酯酶(ES)同工酶、淀粉酶(Am)同工酶进行了研究。在ES同工酶的研究中,九龙牦牛5带较多,占43.9％,麦洼牦牛6带较多,占54.7％;九龙牦牛的带型座含有Es—5,而麦洼牦牛有Es—8,却无Es—5;Am基因座均受Am^A、Am^B、Am^C三个等显性等位基因控制,两个品种都未出现AA和AB型,且Am^c基因频率是两个品种的优势基因;麦洼牦牛的杂合子频率(0.867)高于九龙牦牛(0.780)。表明麦洼牦牛的遗传特性比九龙牦牛复杂。对麦洼牦牛的两个同工酶的活力与产奶量、乳脂率以及体重的关系作了相关分析,结果表明ES同工酶的强带与产奶量、乳脂率呈显著正相关、与体重呈显著负相关。Am同工酶的总活力与产奶量、乳脂率呈显著的负相关、与体重呈显著正相关。这不仅表明了ES同工酶、Am同工酶与生产性能具有较密切关系,而且对开展选育工作及牦牛生产性能鉴定等方面有重要意义。
By the use of disconiinous buffer polyacrylamide-gel eiectrophoresis, blood samples from 50 Jiulong and 50 Maiwa yaks in sichuan province were studied for estesase(Es) and amylase (Am) isozymes. The comparative Es isozyme of study five bands were dominant with the proportion of 43.9％ in Jiulong yaks, There is Es-5; of bandgroup in Jiulong yaks, Mai Wa yaks are Es-8 and not are have Es-5; major bands activities of Jiulong yaks are lower than maiwa yaks'. The comparative Am isozyme in two yak bereeda, the ratioes of heterozygotes of Maiwa yaks (0.867) are higher than Jiulong yaks (0.870) . Genetic characters of Maiwa yaks are more complicated than Jiulong yaks'in more than ever proving, they are different in the two local yak breeds. Activities of two isozymes are comparative with milk yields, fat percentanges and mature sizes in Maiwa yaks, The results showed that Es and Am isoymes are highly significant difference with three productivities. Not only demonstrated two isozymes that were connect highly with productivities but also will promote amelioration in yaks.
出处
《西南民族大学学报（自然科学版）》
CAS
1992年第3期255-260,共6页
Journal of Southwest Minzu University(Natural Science Edition)
关键词
牦牛
ES同工酶
Am同工酶
带型
活力
Es Isozyme, Am Isozyme, Jiulong yak, Maiwa yak, Band group, Activity.
