摘要
NAC转录因子在植物发育和逆境应答中具有重要的作用。本研究从大豆中克隆了1个NAC基因GmNAC115。该基因c DNA全长1 383 bp,开放阅读框长912 bp,编码303个氨基酸,预测分子量约为34.278 k D,p I8.37。推测的氨基酸序列中含有1个高度保守的NAM结构域。Gm NAC115基因组包含3个外显子和2个内含子。进化树分析发现Gm NAC115和Pv NAC为1个分支。转录活性分析结果表明,Gm NAC115转录因子具有转录激活功能。SDSPAGE电泳分析表明,p ET-28a-Gm NAC115最佳诱导表达条件为在37℃下1.0 mmol·L-1IPTG诱导2 h。组织特异性表达模式分析表明在检测的所有组织中Gm NAC115都有表达,在根中表达量最高,在种子中表达量最低。
NAC （NAM, ATAF, and CUC）-domain proteins play important roles in plant development and stress responses. A gene coding for NAC, designated as GmNACll5 was isolated from soybean. The full length GmNAC115 eDNA is 1 383 bp, in- cluding an ORF of 912 bp. This eDNA sequence encoded a polypepide of 303 amino acid residues with a predicted molecular mass of 34. 278 kD and a basic isoelectric point of 8.37, and a highly conserved NAM domain in the encoded putative protein. The genomie DNA of GmNAC115 contains three exons and two introns. Phylogenetic analysis indicated that GmNAC115 clustered with PvNAC. Transcriptional activation ability assay showed that the GmNAC115 transcription factor had transcriptional activation functions. SDS-PAGE indicated that the best expression quantity of the pET-28a-GmNAC115 was induced with 1.0 mmol· L^-1 IPTG for 2 h at 37℃. Tissue-specific expression profiles showed that GmNAC115 expressed almost in all detected tissues, and its expression level was relatively high at root and low at seed.
作者
倪志勇
于月华
陈全家
曲延英
NI Zhi-yong YU Yue-hua CHEN Quan-jia QU Yan-ying(College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, Chin)
出处
《大豆科学》
CAS
CSCD
北大核心
2016年第5期754-759,共6页
Soybean Science
基金
新疆农业大学前期资助课题(XJAU201313)
