摘要
目的探讨不同剂量锌对高糖高脂饮食大鼠胰岛素敏感性的影响。方法健康雄性SD大鼠68只,随机分为正常饲料组(n=29)和高糖高脂组(n=39);喂养17周后正常饲料组和高糖高脂组再分别随机分成5组,分别给予蒸馏水、5、10、15、30 mg/kg锌灌胃,持续7周;测定大鼠血糖、空腹胰岛素(FINS)和糖化血红蛋白水平(HbA1c),计算稳态模型胰岛素抵抗指数(HOMA-IR)和胰岛素敏感指数(ISI)。结果正常饲料组和高糖高脂组大鼠在灌胃前后血糖差异均无统计学意义;与灌胃前比较,锌灌胃后高糖高脂组FINS、HOMA-IR均有不同程度升高(P<0.05),高糖高脂+10、15 mg/kg锌组灌胃后FINS分别为(13.574±2.297)、(11.050±1.654)μIU/mL,均低于其他各组(P<0.05);与灌胃前比较,锌灌胃后高糖高脂组ISI水平有不同程度降低,高糖高脂+15 mg/kg锌组灌胃后ISI水平为(-1.733±0.075),均低于其他各组(P<0.05)。结论锌可在一定程度上提高高糖高脂饲料喂养大鼠胰岛素敏感性,但不同剂量锌效果存在差异。
Objective To investigate the effect of different dose of zinc on insulin Sensitivity in rats fed with high sucrose and fat diet. Methods Totally 68 healthy male Sprague-Dawley rats were randomly allocated to two groups ： normal diet group（ n = 29） and high-sucrose-fat diet group（ n = 39 ）. After 17 weeks, the rats of the two groups were randomly assigned to five groups, each group was administered with distilled water or zinc at dose of 5,10,15 or 30 mg/kg daily by gavage for 7 weeks. Fasting plasma glucose, fasting plasma insulin （FINS） and glycohemoglobin （HbA1 c） were detected. Then the homeostasis model assessment for insulin resistant（HOMA-IR） and insulin sensitively index（ISI） of each group were calculated. Results Compared with normal diet group,the glucose of high-sucrose-fat diet groups was insignificantly different before or after the gavage. FINS and HOMA-IR were significantly increased among high-sucrosefat diet groups after zinc administration（P 〈0. 05） ,while the FINS for the group of 10 mg zinc was 13. 574 ± 2. 297 μIU/ml and that of 15 mg zinc group was 11. 050 ± 1. 654 μIU/ml,both lower than other zinc level groups（P 〈0. 05）. ISI were significantly decreased among high-sucrose-fat diet groups after zinc administration, and that for the group with 15 mg zinc gavage was - 1. 733 ± 0. 07530, significantly lower than that of other groups （ P 〈 0. 05 ）. Conclusion Zinc could at some degree improve insulin sensitivity in rats fed with high-sucrose-fat diet,depending on the dose.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2013年第5期691-693,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(81172665)
天津市自然科学基金(10JCYBJC11100)
关键词
锌
高糖高脂饲料
大鼠
胰岛素抵抗
胰岛素敏感性
zinc
high sucrose and fat die
rat
insulin resistance
insulin sensitivity
