摘要
采用无油磷脂为底物,酸碱滴定法测定磷脂酶LecitaseUltra的酶活力,在单因素试验的基础上,选定反应时间、反应温度、反应pH值进行响应面分析试验,建立磷脂酶酶活力的二次回归方程,通过响应面分析得到酶活力测定的优化组合条件。结果表明:反应时间、反应温度、反应pH值对酶活力都有极其显著影响,测定酶活力的最佳条件为:底物质量浓度4g/100mL、终点pH 9.0、酶液稀释倍数100倍、反应pH5.1、反应温度53℃、反应时间8min,得到磷脂酶的酶活力为9550U/g。
To establish a method to determine the activity of phospholipase Lecitase~ Ultra using oil-free phospholipids as the substrate, three reaction parameters including time, temperature and pH were optimized by response surface methodology based on one-factor-at-a-time experiments. A quadratic regression model for phospholipase activity was proposed. The results showed all these parameters had an extremely significant effect on phospholipase activity. The optimal conditions for determin- ing phospholipase activity required substrate concentration of 4 g/100 mL, final pH 9.0, enzyme dilution factor of 100, reaction pH 5.1, reaction temperature of 53 ℃ and reaction time of 8 min.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第17期174-178,共5页
Food Science
基金
"十二五"国家科技支撑计划项目(2011BAD02B02)
安徽省科技攻关计划项目(2011AKKG1506)
