摘要
目的了解单纯雪旺细胞（ｓｃｈｗａｎｎ．ｃｅｌｌ， ＳＣ）移植或附加胚胎脊髓细胞（ｆｅｔａｌｓｐｉｎａｌｃｏｒｄｃｅｌｌ， ＦＳＣ）对中枢轴索再生的作用。方法ＳＣ悬液（ｓｃｈｗａｎｎｃｅｌｌｓｕｓｐｅｎｓｉｏｎ，ＳＣＳ）单独和与ＦＳＣ悬液（ｆｅｔａｌｓｐｉｎａｌｃｏｒｄｃｅｌｌｓｕｓｐｅｎｓｉｏｎ，ＦＳＣＳ）联合植入成鼠胸段（Ｔ）打击伤１周脊髓空腔中，相同量的Ｄｕｌ－ｂｅｃｃｏ改良细胞培养液（ｄｕｌｂｅｃｃｏ＇ｓｍｏｄｉｆｉｅｄｅａｇｌｅ ｍｅｄｉｕｍ，ＤＭＥＭ）植入作为对照。移植后２、６周进行ＨＥ、嗜银染色组织学观察，免疫组化髓鞘碱性蛋白（ｍｙｅｌｉｎｂａｓｉｃ ｐｒｏｔｅｉｎａｎｔｉｂｏｄｙ，ＭＢＰ）染色观察ＳＣ及髓鞘，５－羟色胺（５－ｈｉｓｔａｍｉｎｅａｎｔｉｂｏｄｙ，５－ＨＴ）染色观察下行运动纤维；神经丝（ｎｅｕｒｏｆｉｌａｍｅｎｔａｎｔｉｂｏｄｙ，ＮＦ）染色观察ＦＳＣ向神经元的分化。结果在宿主中ＳＣ存活、增殖，ＦＳＣ分化趋向成熟神经元。含ＳＣ的移植物中均见再生长入的宿主轴索，且多与束状排列的ＳＣ相伴随，二者ＭＢＰ染色均为阳性。混合移植物中，５－ＨＴ阳性纤维相对丰富，对照组未见再生轴索。结论自成鼠周围?
Objective To observe the effect of Schwann cells suspension (SCS) grafts either aloneor mixed with fetal spinal cord cells suspension (FSCS) on axonal regeneration of central nervous system.Methods The SCS and SCS+FSCS were implanted into the empty space left following injured thoracic (T7)spinal cord injury in adult rats one week later, while the vehicle solution-DMEM was grafted in the controls.At 2 and 6 weeks post-transplantation, the samples were procured and studied histologically with silver and HEstains. In immunohistochemical assay, myelin basic protein antibody (MBP)was used to identify Schwann cells(SC) and myelin, serotonin antibody (5-HT), a specific neurotransmitter to raphe-derived axons, was usedfor descending motor fibere, and neurofilament antibody (NF) was used for the immature neurons derived fromgrafts. Results SC could survive and proliferate, FSC could differentiate in the host spinal coal, regrowingaxons in grafts were associated with bundles of SCs, both axons and SCs were immunoreative for MBP, 5-HTpositive nerve fibers were relatively abundant in SCS plus FSCS grafts. There was no neurite in DMEM grafts.Conclusion SCS cultured from adult rat peripheral nerves was able to promote spinal axonal regenerationand to construct the myelin sheath, the mixed grafts could stimulate regrowing axons derived from somesupraspinal neurons regenerate into the grafts at the thoracic level in the adult rat spinalcord.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1999年第2期77-79,I001,共4页
Chinese Journal of Orthopaedics
