摘要
本文针对脂肪酶LecitaseUltra的特殊性(同时具有脂肪酶活性和磷脂酶活性),采用无油磷脂为底物的酸碱滴定法测定LecitaseUltra的磷脂酶酶活,探讨了测定中的几个关键因素对酶活测定值的影响,建立了一套快捷可靠的磷脂酶酶活测定方法。
A method for measurement of the phospholipase A1 activity by acid-base titration with deoiled phospholipid as substrate was adopted in this paper, according to the characteristic of lipase Lecitas Ultra which had both high lipase and phospholipase activity, The effect of some key factors on the measurement of enzymatic activity was evaluated, and a rapid and reliable method for measuring the phospholipase A1 activity of Lecitas Ultra was established.
出处
《现代食品科技》
EI
CAS
2007年第8期80-82,共3页
Modern Food Science and Technology