摘要
应用染色体原位杂交、ＰＣＲ扩增、克隆及核苷酸序列分析等方法，分析了ＣＮＥ１和ＣＮＥ３细胞株中的潜伏感染膜蛋白（ＬＭＰ１）基因。ＣＮＥ１是来自我国东北的高分化鼻咽癌细胞株，ＣＮＥ３是来自广西的低分化鼻咽癌细胞株。染色体原位杂交结果表明，ＣＮＥ１细胞中ＬＭＰ１基因存在于细胞核内，整合在第一号染色体上，ＣＮＥ３中ＬＭＰ１基因则随机存在于细胞核内及多条染色体上。用ＰＣＲ方法分别从ＣＮＥ１及ＣＮＥ３中扩增得到了ＬＭＰ１基因片段（外显子３），核苷酸序列分析证明，来自ＣＮＥ１的ＬＭＰ１与来自Ｂ９５－８细胞的ＬＭＰ１核苷酸序列同源性极高，达９９．５％，而ＣＮＥ３的ＬＭＰ１基因与Ｂ９５－８的ＬＭＰ１基因同源性为９３％。
he latent membrane protein (LMP1)gene of Epstein-Barr Virus EBV)was found inCNE1,a well differentiated nasopharyngeal carcinoma (NPC)cell line from northeast of China,and in CNE3,a poorly differentiated NPC cell line came from Guangxi Province of China. The results of in situ hybridizatioon showed that LMP1 gene is integrated into chromosome No.1 inCNE1, and into severdl chromosomes in CNE3.The exon 3 of LMP 1 gene was amplified byPCR and cloned from CNE1 and CNE3.Sequence analyses of these PCR products revealedthat there is a high homology of 99.5% between B95-8strain and CNE1,and a lowerhomology of 93% between B95-8and CNE3.
出处
《病毒学报》
CSCD
北大核心
1995年第2期114-118,共5页
Chinese Journal of Virology
关键词
EB病毒
潜伏感染膜蛋白
原位杂交
鼻咽肿瘤
Naso pharyngeal carcinoma
Epstein-Barr virus
Latent membrane protein In situ hybridization
PCR
Cloning
Sequencing
