摘要
目的研究转化生长因子β(TGFβ)在狼疮肾炎中的作用。方法比较BXSB狼疮小鼠和BALB/c小鼠24h尿蛋白总量,并用逆转录聚合酶链反应技术(RTPCR)和免疫组织化学方法检测两组小鼠肾组织TGFβmRNA及蛋白表达情况。结果BXSB小鼠24h尿蛋白总量明显高于BALB/c小鼠(P<0.01);RT-PCR检测到BXSB小鼠肾组织TGFβmRNA表达较BALB/c小鼠明显升高(P<0.01);免疫组织化学表明BALB/c小鼠仅在少数肾小管有微弱TGFβ表达,而BXSB小鼠肾小球、肾小管及间质均有明显TGFβ蛋白表达。结论TGFβ可能参与介导了狼疮肾炎。
Objective To investigate the relationship between TGFβ over-expression and lupus nephritis(LN) in BXSB mice.Methods Proteinuria production of two groups was evaluated respectively.The expressions of TGFβ in the kidneys of 6 BXSB mice and 6 age- and sex- matched BALB/c mice were detected by means of RT-PCR and immunohistochemistry technique.Results 24h total amount of urine protein of BXSB mice was higher than that of BALB/c mice (P<0.01).There was a significant stronger expression of TGFβ in the kidney of BXSB mice than that of BALB/c mice(P<0.01). Conclusion The over-expression of TGFβ in the kidney may play a role in mediating LN.
出处
《江苏医药》
CAS
CSCD
北大核心
2005年第6期445-447,F002,共4页
Jiangsu Medical Journal
