摘要
本文建立了测定大鼠腹腔巨噬细胞产生白三烯B_4的反相高效液相色谱法,并初步探讨了白芍总甙对白三烯B_4产生的影响。结果表明,在腹腔巨噬细胞(10~9～15×10^(10)/L)范围内,随着细胞数目的增加,白三烯B_4产生量也增加;白芍总甙(100mg/L)对大鼠巨噬细胞产生白三烯B_4的抑制作用与相同剂量的非甾体抗炎药氟灭酸相当,但作用较缓慢。白芍总甙(0.001～100mg/L)可剂量依赖性地抑制白三烯B_4的产生,其50%抑制率(IC_50)为0.66mg/L。提示白芍总甙的抗炎与免疫调节作用可能与其影响白三烯B_4的产生有关。
Total glucosides of paeony ( TGP ) , an extract of Paeonia Lacti-flora Pall were investigated for its effect on the synthesis of leukotriene B_4 ( LTB_4 ) by peritoneal macrophages ( PMφ). LTB4, generated by 10~9~15 × 10~10/L PMφ with ionophore A_23187 , were measured with RP-HPLC. Suppression of releasing LTB_4 from PMφ by TGP ( 100 mg/L ) was as much as the same dose of Flufenamic acid, but the action of TGP was slower. TGP significantly inhibited the synthesis of LTB_4 by PMφ in a dose-dependent manner. The concentration of TGP required to obtain 50% inhibition ( IC_50 ) of formation of LTB_4 was 0.66mg/L. The data suggested antiinflam-matory and immunomodulatory action of TGP were related to its suppression of releasing LTB_4
出处
《中国药理学通报》
CAS
CSCD
北大核心
1992年第1期36-39,共4页
Chinese Pharmacological Bulletin
关键词
白三烯B4
白芍
总甙
高效液相色谱
Leukotriene B_4
Paeonia Lactiflora Pall
Total gluco-sides of paeony
Peritoneal macrophages
RP-HPLC
