摘要
目的建立高效液相色谱方法测定血浆中莫西沙星的浓度,为耐多药结核患者中应用莫西沙星的临床安全合理用药提供参考。方法人血浆经甲醇沉淀蛋白后,采用高效液相-紫外色谱法测定。色谱柱为岛津Inertsustain C 18柱,流动相为甲醇-醋酸盐缓冲液(38∶62),流速为1.0mL·min-1,柱温为45℃,检测波长为295nm,进样体积为20μL。结果莫西沙星的保留时间约为11.3min,血浆内源物质对测定无干扰;莫西沙星血药浓度在0.1~10.0μg·mL-1范围内线性关系良好;最低定量限浓度为0.1μg·mL-1;绝对回收率为78.02%~84.09%,相对回收率为80.01%~111.11%,日内、日间精密度的RSD均<16.06%。结论该测定方法具有操作简单,回收率高,结果准确等优点,可以为临床合理安全用药提供依据。
OBJECTIVE To establish the method for the determination of plasma concentration of moxifloxacin,to further evaluate the clinical safety of the MDR-TB patients treated with moxifloxacin.METHODS Normal plasm a was pretreated with methanol for protein precipitation and determined by HPLC-UV;Column:Inertsustain C 18 column(4.6mm×250mm,5μm);Mobile phase:methanol-buffered solution(38∶62),the buffer solution was an acetic acid-sodium acetate buffer solution with pH 4.70;Flow rate:1.0mL·min-1;Column temperature:45℃;Detection wavelength was 295nm;Injection volume:20μL.RESULTS The retention time of moxifloxacin was 11.3min,which showed a good shape,and was not interfered with the determination by plasma endogenous peak.The plasma concentration ranged from 0.1 to 10μg·mL-1.The minimum quantitative concentration was 0.1μg·mL-1,the absolute recoveries were 78.02%-84.09%,and relative recoveries were 80.01%-111.11%,and RSDs of intra-day and inter-day were less than 16.06%.CONCLUSIONThe method is simple and high recovery rate of extraction and high accura cy.It could be the reference of the clinical safety medication.
作者
张婷婷
卢弢
徐然
ZHANG Ting-ting;LU Tao;XU Ran(Department of Pharmacy,The Second Hospital of Nanjing,Nanjing University of Chinese Medicine,Nanjing Public Health Center,Nanjing 210003,China;Nanjing Institute for Food and Drug Control,Nanjing 211100,China)
出处
《海峡药学》
2019年第11期62-65,共4页
Strait Pharmaceutical Journal
