摘要
线粒体作为重要的细胞器,在植物进化适应过程中起着不可忽视的作用。抗旱耐寒常绿阔叶灌木沙冬青主要分布在我国西北荒漠地区,是第三纪孑遗植物,研究该植物线粒体基因组与长期进化适应的关系具有重要意义。然而,用常规的方法难以分离纯化获得沙冬青线粒体基因组DNA,限制了沙冬青线粒体功能基因组研究。该研究利用不连续蔗糖梯度密度离心和DNase I消化技术,成功获得了高纯度沙冬青线粒体DNA,该线粒体DNA的纯度和完整性满足建库的要求,为沙冬青线粒体基因组研究提供了技术支持,对其他植物线粒体基因组学研究也具有参考价值。
Mitochondria is essential in the process of plant evolution. Xerophyte evergreen broadleaf shrub Ammopiptanthus mongolicus is a tertiary relic plant, which is mainly distributed in the desert region of northwest China. Analyses of the complete mitochondrial genome of A. mongolicus may provide valuable information for understanding the evolutionary adaptation of this xerophyte. However, it is difficult to isolate and purify the mitochondrial genome DNA of A. mongolicus using the conventional method. So, researches on A. mongolicus mitochondria function genome is restricted. In this study, we successfully obtained the high purity mitochondrial DNA by using the discontinuous sucrose density gradient centrifugation and DNase I digestion technology. The mitochondrial DNA purity and integrity met the requirements of building library for mitochondrial genome sequencing, supplying technological support for A. mongolicus functional mitochondria genome research.
作者
于婷乔
崔洪玮
孙丽春
刘胜利
陈玉珍
卢存福
Yu Tingqiao;Cui Hongwei;Sun Lichun;Liu Shengli;Chen Yuzhen;Lu Cunfu(Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beij＇ing 100083, China)
出处
《中国细胞生物学学报》
CAS
CSCD
2018年第3期390-396,共7页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:31270737)
高等学校学科创新引智计划(批准号:B13007)
长江学者和创新团队发展计划(批准号:IRT13047)
北京市自然科学基金(批准号:6112016)资助的课题~~
关键词
沙冬青
线粒体DNA
提取纯化
质量检测
Ammopiptanthus mongolicus
mitochondrial DNA
extraction and purification
quality detection
