摘要
从凡纳滨对虾中分离和鉴定腐败优势菌,并对其进行群体感应信号分子AHLs和AI-2的测定。通过细菌自动生化鉴定系统和16S r DNA序列鉴定优势腐败菌;利用生物检测菌根癌农杆菌(Agrobacterium tumefaciens)JZA-1测定其AHLs、哈维弧菌(Vibrio harveyi)BB170检测其AI-2,并探究其动态生成规律。最终分离得到10株腐败优势菌,有4株菌均为变形杆菌属(Proteus),其他分别为Oceanisphaera profunda、溶血性葡萄球菌(Stapylococcus haemolyticus)、腐败希瓦氏菌(Shewanella putrefaciens)、短杆菌属(Brevibacterium)、Bacillus siamensis、Exiguobacterium aaestuarii。分离腐败优势菌中的6株革兰氏阴性菌均产生AHLs信号分子;变形杆菌属、腐败希瓦氏菌、短杆菌属、Bacillus siamensis产生AI-2信号分子。优势腐败菌的AHLs活性与菌体生长密度呈正相关,AI-2产生量在菌体生长对数期累积,在菌体生长24 h后活性迅速减弱。
This study was aimed at isolating,identifying the dominant spoilage bacteria from Litopenaeus vannamei using an automatic biochemical identification system and 16 S r DNA sequence,detecting the quorum sensing signaling molecules acyl homoserine lactones（AHLs）,autoinducer-2（AI-2）and exploring the dynamic formation regularities with indicator bacteria Agrobacterium tumefaciens JZA-1 and Vibrio harveyi BB170,respectively.Ten strains were selected and identified,including Oceanisphaera profunda,Stapylococcus haemolyticus,Shewanella putrefaciens,Brevibacterium sp.,Bacillus siamensis,Exiguobacterium aaestuarii and four strains of the Proteus genus.All the Gram-negative spoilage organisms isolated secreted signaling molecule AHLs while Proteus sp.,Shewanella putrefaciens,Brevibacterium sp.and Bacillus siamensis secreted signaling molecule AI-2.The AHLs activity of dominant spoilage organism was positively correlated with cell density.The AI-2 production was accumulated within the logarithmic phase and decreased rapidly from 24 h onwards.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第8期164-169,共6页
Food Science
基金
国家自然科学基金面上项目(31371746
31201309
31171634)
“十二五”国家科技支撑计划项目(2012BAD29B06)
广东省高等学校创新强校项目(GDOU2013050312
GDOU2013050205
GDOU2013050203)
关键词
凡纳滨对虾
优势腐败菌
群体感应
信号分子
Litopenaeus vannamei
dominant spoilage organism
quorurm sensing
signaling molecule
