摘要
为研究UFGT基因对红皮梨花色素苷合成的调控机制,以云南特有火把梨为试材,克隆得到UFGT的基因片段,运用生物信息学分析软件对该基因序列进行分析。结果表明:UFGT基因片段长1 440 bp,编码479个氨基酸的蛋白质;该氨基酸序列与沙梨、西洋梨、白梨、苹果和樱桃李的同源性分别为99%、96%、94%、91%和72%;蛋白无跨膜结构,不存在导肽和信号肽,属于非分泌蛋白,定位在胞液;结构功能域分析显示该片段含典型的GT1_Gtf_Like功能域和1个GTB型超家族蛋白的典型结构。
In order to research UFGT gene for regulation mechanism of anthocyan synthesis in red pear,Pyrus pyrifolia cv. Huobali of specific in Yunnan was used as experimental material and the cDNA fragment ofUFGT gene was obtained by homologous cloning. Then, bioinformatics analysis of UFGT gene and its deducedprotein was done by using the related biological software. The results showed that length of UFGT is 1 440 bp,and it encodes 479 amino acids. The homology at amino acids level of UFGT with UFGT in Pyrus pyrifolia（AFQ92055）, Pyrus communis （ AGLS1353 ）, Pyrus bretschneideri （ AGZ15304 ）Malus domestica （ XP_008357063） and Prunus cerasifera （AKV89253） are 99%, 96%, 94% ,91% and 72%, respectively. The re-suits of amino acid sequence analysis indicated that the UFGT had no signal peptide and trans-membrane domain,belonged to the non secreted protein and located in cytosol. The protein predication of UFGT showed that therewere a function domains GT1 Gtf Like and typical structure of GTB super family.
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2016年第1期21-27,43,共8页
Journal of Southwest Forestry University：Natural Sciences
基金
云南省教育厅科学研究基金项目(2012J050)资助
西南林业大学科研启动基金资助
关键词
火把梨
UFGT
基因克隆
生物信息学
Pyrus pyrifolia cv. Huobali
UFGT
gene cloning
bioinformatics
