摘要
选取青岛市5个功能区(市区街道、海滨区域、饮用水源地、垃圾填埋场和人工湿地污水处理系统),采用SAS ISO100空气浮游菌采样器于2013年冬季采集空气微生物样品,应用BIOLOG方法分析空气微生物群落代谢功能多样性,阐明群落代谢与环境相关性。结果表明,不同功能区空气微生物群落碳源代谢强度存在差异,代谢稳定时,海滨区域和饮用水源地样品平均光密度值(AWCD)分别为0.302、0.210,而人工湿地、市区街道及垃圾填埋场分别为0.063、0.025和0.034,海滨区域和饮用水源地空气微生物群落碳源代谢强度明显高于其他功能区。不同功能区空气微生物群落Shannon指数和Simpson指数接近,但海滨区域和饮用水源地Mc Intosh指数明显高于其他功能区。海滨区域和饮用水源地空气微生物群落碳源代谢类型丰富,代谢水平高,人工湿地、市区街道和垃圾填埋场碳源代谢类型单一,代谢水平低。5个功能区空气微生物群落碳源代谢差异呈现区域性,分异代谢差异的主要是羧酸类碳源。风速、温度、湿度等非生物因素对空气微生物群落碳源代谢具有不同程度影响,且不同功能区主导非生物因素存在差异。BIOLOG方法可以提供大量多维数据,能够分析样品间微生物群落碳源代谢差异,客观、全面表征空气微生物群落碳源代谢多样性特征,是研究空气微生物群落功能多样性较理想的方法之一。
Airborne microbes are major biological components of the ecosystem that have important ecosystem functions, affect human health and air pollution, and are key indicators of air quality. To determine the metabolic characteristics and community diversity of airborne microbes in different functional regions of Qingdao in winter, sampling sites were set up in five different functional regions （ urban streets, a coastal area, drinking water source area, municipal landfill, and artificial wetlands）. Airborne microbes were then collected using an SAS ISO100 air sampler in January 2013 and their carbon source metabolic characteristics, functional diversity, and relationship with environmental factors were systematically analyzed by the BIOLOG method. The results revealed significant differences in carbon metabolic profiles of air microbial communities from the five locations. When metabolism was stable, the average well color developments of samples from the coastal area and drinking water source area were 0.302 and 0.21, respectively, whereas those of artificial wetlands, urban streets, and municipal landfill were 0.063, 0.025, and 0.034, respectively. Therefore, the levels of carbon metabolism in the coastal area and drinking water source area were higher than those at other locations. The Shannon indexes and Simpson indexes at the five different source area were functional regions were similar. However, the McIntosh indexes at the coastal area and drinking water higher than those at other locations. Overall, the microbial diversity and dominant species differed among functional regions, but microflora was distributed more evenly in the coastal area and drinking water source area. Among the five locations, carbon catabolic types and levels in the coastal area and drinking water source area were richer and higher than those in the artificial wetlands, urban streets, and municipal landfill. Overall, the airborne microbes in various functional areas had high carbohydrate and carboxylic acid metabolism, and air microb
出处
《生态学报》
CAS
CSCD
北大核心
2015年第7期2277-2284,共8页
Acta Ecologica Sinica
基金
国家自然科学基金项目(31170509)
关键词
青岛市
城市功能区
空气微生物
代谢特征
群落多样性
BIOLOG方法
Qingdao City
city functional regions
airborne microbial community
metabolic characteristics
microbialdiversity
BIOLOG method
