摘要
研究了大黄素在低氧肠应激损伤中通过细胞自噬机制如何起保护作用.以IEC-6细胞为研究对象,采用噻唑蓝染色法(MTT)筛选出最佳大黄素浓度.在常氧(氧浓度20.9%)及低氧环境下(氧浓度5%)6h、24h后,用噻唑蓝染色法(MTT)检测其细胞存活率;采用流式细胞仪检测细胞周期,并通过实时荧光定法(Real-Time PCR)检测自噬相关基因Beclin-1及LC3的表达.噻唑蓝染色法(MTT)结果表明,与常氧对照组相比,在低氧条件下无大黄素组细胞生存率明显降低(P<0.01),而大黄素组细胞生存率无明显差异(P>0.05);流式细胞仪结果表明,相对于无大黄素组,大黄素组可明显促进细胞进入分裂期(P<0.01);Real-Time PCR结果表明,在低氧6h、24h后大黄素组Beclin-1及LC3基因表达量均上调(P<0.05).可知在低氧肠应激性损伤下,大黄素通过自噬机制起保护作用.
Research on emodin in hypoxia stress damage,which protect the cell through the autophagy system.IEC-6cells as the research object,selects the best concentration of emodin by MTT method,respectively under the constant oxygen（20.9% oxygen concentration）and low oxygen（5% oxygen concentration）after 6hand 24 h,tests cell survival rate by MTT,detects the cell cycle using flow cytometry instrument,and tests autophagy related genes by Real Time-PCR.MTT results suggest：compared with the control group,under the condition of low oxygen without emodin group cell survival rate was significantly lower（P0.01）;No significant difference was observed between the control group and emodin group（P0.05）.Flow cytometry results suggest：emodin group can obviously promote cell into the split phase（P0.01）.Real-Time PCR results suggest：at low oxygen after 6hours and 24 hours,the expressions of Beclin-1and LC3 gene expression increased significantly（P0.05）in emodin group.In hypoxia stress damage,emodin may as a protector through the autophagy system.
出处
《陕西科技大学学报（自然科学版）》
2015年第2期134-138,共5页
Journal of Shaanxi University of Science & Technology
基金
甘肃省科技厅杰出青年科技基金项目(1111RJDA004)
关键词
自噬
低氧
肠应激性损伤
大黄素
保护
autophay
hypoxia
intestinal stress damage
emodin
protection
