摘要
为了解精液中不同类型糖类物质对精子细胞中N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)的影响,以莆田黑猪精液NAGase为材料,研究精液和精液稀释液主要成分之一果糖对酶的抑制作用.结果显示,果糖对NAGase具有浓度效应(IC50=575 mmol L-1).果糖对NAGase的抑制类型是可逆的非竞争性抑制,抑制常数是1.27×10-2 mmol L-1.应用底物反应的动力学方法研究果糖对该酶的抑制动力学,建立了抑制动力学模型,证明了果糖对该酶的影响是快速结合再缓慢失活的过程.本研究表明,在高浓度的抑制剂溶液中NAGase将完全失活,底物对其无保护作用.
This research aimed to understand the effects of different types of sugar on the N-Acetyl-β-D-glucosaminidase（EC 3.2.1.52,NAGase） in sperm cells.NAGase from semen of Putian black pigs was used as the material to study the effects of fructose,one of the main components of the sperm and semen dilutions.The inhibition kinetics of fructose on the enzyme model was set up by using the kinetic method of substrate reaction.The results showed that NAGase was dose-dependent on fructose（IC50= 575 mmol L^-1） and the inhibition type of fructose on NAGase was reversible noncompetitive inhibition,with the inhibition constant as 1.27×10^-2 mmol L^-1.The process of fructose on the enzyme was first slow deactivation then rapid combination.These results suggested that the enzyme is completely deactivated in fructose solution of high concentration,where the substrate has no protection to the enzyme.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2015年第1期46-50,共5页
Chinese Journal of Applied and Environmental Biology
基金
福建省科技计划项目重点项目(2012N0002)资助~~
