摘要
目的:人参多糖注射液对胃癌细胞SGC-7901凋亡的诱导作用及机制.方法:采用不同浓度(0、12.5、25.0、50.0、100.0μL/m L)、不同时间(24、48、72 h)人参多糖注射液处理SGC-7901细胞,分别采用流式细胞仪及Western blot检测细胞凋亡及Bax、Bcl-2蛋白表达.结果:人参多糖注射液对SGC-7901细胞体外凋亡具有显著的促进作用,并呈现剂量及时间依赖性.人参多糖注射液处理后,SGC-7901细胞Bax蛋白表达及Bax/Bcl-2蛋白表达比值显著升高,Bcl-2蛋白表达显著降低,并呈现剂量及时间依赖性.结论:人参多糖注射液可诱导胃癌SGC-7901细胞凋亡,调节凋亡相关基因Bax、Bcl-2表达是其可能作用机制.
AIM： To investigate the apoptosis-inducing effect of ginseng polysaccharide injection in gastric carcinoma SGC-7901 cells and the possible mechanism involved. METHODS： SGC-7901 cells were treated with different concentrations（0, 12.5, 25.0, 50.0, 100.0μL/m L） of ginseng polysaccharide injection for different durations（24, 48 or 72 h）. Cell apoptosis was assessed by flow cytometry, and protein expression of Bax and Bcl-2 was determined by Western blot.RESULTS： Ginseng polysaccharide injection significantly induced the apoptosis of SGC-7901 cells in vitro in a dose- and timedependent manner. Treatment with ginseng polysaccharide injection significantly in-creased Bax protein expression and Bax/Bcl-2 ratio, and reduced Bcl-2 protein expression in a dose- and time-dependent manner. CONCLUSION： Ginseng polysaccharide injection is able to induce apoptosis of gastric carcinoma SGC-7901 cells possibly by regulating Bax and Bcl-2 protein expression.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第33期5114-5117,共4页
World Chinese Journal of Digestology
关键词
人参多糖注射液
胃癌
凋亡
BAX
BCL-2
Ginseng polysaccharide injection
Gastric carcinoma
Apoptosis
Bax
Bcl-2
