摘要
目的 :通过观察大鼠肾脏缺血再灌注 ( I-R)的不同时间 IL-6和 TNF-α的变化 ,探索细胞因子在肾脏 I-R损伤中的作用。方法 :采用大鼠肾脏 I-R损伤模型 ,分别在缺血和再灌注 1、4和 2 4 h时取血和肾组织 ,用双抗体夹心 ELISA法测定 IL-6和 TNF-α的含量。结果 :缺血组及再灌注 4 h( R4 h)和 2 4 h( R2 4 h)组 IL-6含量均低于对照组 ( P<0 .0 5 ) ;而再灌注 1 h组与对照组相当 ,但显著高于缺血组 ( P<0 .0 1 )。IL-6含量的动态变化在肾组织和血液中呈直线正相关 ( P<0 .0 5 )。肾组织中 TNF-α含量在缺血和再灌注 1 h组均高于对照组 ( P<0 .0 5 ) ,血液中 TNF-α与对照组无显著性差异。结论 :大鼠肾脏 I-R时肾组织和血液中 IL-6含量随时间的推迟而降低 ,而肾组织中
Objective:To explore the effects of cytokines on renal ischemia reperfusion(I R) injury.Methods:The model of rat renal I R injury was prepared and IL 6 and TNF α contents were determined by using enzyme linked immunosorbent assay(ELISA).The renal coefficients,serum urea nitrogen(SUN)and serum creatinine(SCr)were also measured.Results:The contents of IL 6 in ischemia group and reperfusion 4(R4h) and 24 hours(R24h) groups were lower than that in control group,and the level of IL 6 was similar in reperfusion 1 hour group and control group but higher in ischemia group( P<0 01 , P<0 05 ).Linear positive correlation was found between blood and renal IL 6 changes in different stages( P<0 05 ).The concentrations of TNF α in renal tissues were obviously high after ischemia and reperfusion 1 hour( P<0 05 ) in control group,but no changes were found in blood.Conclusion:The contents of IL 6 changed with time and TNF α increased only in the early stages of rat renal I R injury.
出处
《滨州医学院学报》
2002年第1期1-3,共3页
Journal of Binzhou Medical University
