摘要
目的 :建立山羊颞下颌关节损伤模型,比较不同损伤类型的组织病理学改变及对预后的影响。方法 :模拟临床损伤类型,16只成年山羊双侧关节随机分为4组。DD组:剪断关节盘后附着,牵引向前,固定于冠突根部,形成不可复性关节盘前移位;CCD组:磨除髁突外侧1/3软骨,造成髁突软骨损伤;DD+CCD组:形成关节盘前移位+髁突软骨损伤。C组:空白对照组。分别于术后2周和1个月观察3种损伤类型的大体及组织学改变。对髁突软骨进行组织学评分,采用SPSS17.0软件包比较4组之间的差异。结果:DD+CCD组,2周和1个月时见囊内纤维性黏连,剩余软骨粗糙;镜下见剩余软骨结构紊乱,蛋白多糖降解。1个月时,软骨剥脱及囊内纤维黏连加重。DD组,2周和1个月时剩余髁突软骨结构紊乱,纤维带完整,少量血管再生;蛋白多糖少量降解。CCD组,2周和1个月时缺损区与关节盘发生黏连;剩余软骨结构和蛋白多糖与空白对照组相比基本正常。各组之间比较,软骨退行性变程度1个月时比2周时更严重,统计评分按严重程度分别为DD+CCD组(11.75±0.75)、DD组(8.75±0.25)、CCD组(1.75±0.85)和C组(0.00±0.00),各组之间比较均存在显著差异(P<0.05)。结论:通过手术成功建立了3种山羊TMJ损伤模型,其中,DD+CCD组对关节软骨的破坏最严重,DD次之,CCD最轻。
PURPOSE： To establish traumatic temporomandibular joint model in goat, and evaluate the histopathological changes. METHODS： According to the clinical traumatic type, 16 adult goats＇ bilateral joints were randomly divided into 4 groups following surgical procedures. Disc displacement without reduction （DD）： the attachment of disc was severed, and pulled anteriorly; the disc was fixed in the root of coronoid process. Condylar cartilage defect （CCD）： the inferior compartment of TMJ was open to grind off lateral 1/3 condylar cartilage. Disc displacement and condylar cartilage defect （DD＋CCD）： the above two methods were used. Blank control group （C）： nothing was done in TMJ. The TMJs were removed and observed 2 weeks and 1 month after surgery. The histopathological score of condylar cartilage was observed and was statistically analysed between each two groups （4 groups） with SPSS 17.0 software package. RESULTS： DD＋CCD showed fibrous adhesion and coarse condylar cartilage; histologically, the composition of condylar cartilage was in disorder; and matrix proteoglycan decreased at 2-week and 1-month. Partial cartilage desquamation and fibrous adhesion exacerbated at 1-month. DD showed disordered cartilage composition, intact superfacial zone, little angiogenesis and proteoglyean degradation at 2-week and 1-month. CCD showed fibrosis between disc and defective cartilage in defective condyle, and the renmant cartilage composition and proteoglycan were normal at 2-week and 1-month. Cartilage degeneration was more serious at 1-month than 2-week in each group; and cartilage score was significantly different （P〈0.05）in the following order： DD ±CCD （ 11.75 ±0.75 ）,DD （8.75 -±0.25 ）,CCD （ 1.75 ±0.85 ）,and C （0.00 ±0.00）. CONCLUSION： Three traumatic TMJ models in goat were successfully established. The degeneration of cartilage in DD＋CCD was most severe, and CCD was the mildest.
出处
《中国口腔颌面外科杂志》
CAS
2014年第1期13-19,共7页
China Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金(81070848)
国家留学基金委员会回国人员科研启动基金
上海市科学技术委员会资助项目(08DZ2271100)
上海高校创新团队发展计划~~
关键词
颞下颌关节
关节盘前移位
髁突软骨损伤
创伤性骨关节炎
动物模型
Temporomandibular joint
Disc displacement
Condylar cartilage defect
Traumatic osteoarthritis
Animal model
