摘要
目的探讨二至百合浓缩膏对去势雌性大鼠(双侧卵巢切除术)焦虑行为学的影响。方法 3～4月龄Wistar雌性大鼠随机分成5组,假手术对照组,模型组,二至百合高、中、低剂量组。除假手术组,其余用手术摘除双侧卵巢建立去势更年期大鼠模型,给药组分别给予二至百合浓缩膏水溶液(1.92、0.96、0.48g/kg),假手术空白对照组和模型对照组给予相应容量的蒸馏水,连续45d。采用高架十字迷宫和明暗箱实验,观察实验大鼠行为学的变化。结果二至百合高剂量组可显著减低模型大鼠高架十字迷宫实验中OT%,与模型组比较差异有统计学意义(P<0.05)。二至百合高、中剂量组亦可显著升高大鼠明箱停留时间和穿箱次数,与模型组比较差异有统计学意义(均P<0.05)。结论二至百合浓缩膏具有较好的改善去势雌性模型大鼠焦虑症状的作用。
Objective To study the effects of Erzhibaihe extraction on the anxiety behavior of the female castrated rats. Methods 3-4 month-old female wistar rats were randomized into five groups with pseudo-operated group,model group,erzhibaihe high dose group, Medium dose group and low dose group. All rats were established the models of climacteric syndrome with ovariectomized surgical except pseudo-operated group. Treatment group were given Erzhibaihe extraction （ 1.92,0.96,0.48 g/kg）for 45 days, pseudo-operated group and model group rats were given corresponding volumes of distilled water.Then the elevated plus-maze test and light-dark transitions test were performed to observe behavior changes of rats. Results Compared with pseudo-operated group,the OE% （ open arm entry percent ） and OT% （ open arm time percent ） of model group rats were significantly reducedin the elevated plus-maze test.The OT% of Erzhibaihe high dose group were significantly reduced,and there were significantly differenceswith model group.In the light-dark transitions test,the duration in light box and the passing times of model control group were significantly reduced compared with pseudo-operated group（P 〈 0.05）.Compared with model group,the high dose of erzhibaihe extraction could significantly increase the duration in light box and the passing times （P 〈 0.05）,the medium dose of erzhibaihe extraction could also significantly increase the duration in light box and the passing times（P 〈 0.05 ）. Conclusion Erzhibaihe extraction has certain antianxiety effects on the female castrated rats.
出处
《中国医药科学》
2013年第12期20-22,共3页
China Medicine And Pharmacy
基金
国家高技术研究发展计划(863计划)课题(2010AA023006)
关键词
二至百合浓缩膏
抗焦虑
雌性大鼠
去势
高架十字迷宫
明暗箱
Erzhibaihe extraetion
Antianxiety
Female rats
Castrate
Elevated plus-maze
Light-dark transitions
