摘要
目的观察大鼠脑缺血-再灌注(CI/RP)后VEGF和Survivin表达规律及阿司匹林(ASA)对其表达的影响,探讨阿司匹林的神经保护机制。方法健康雄性SD大鼠60只,随机分为对照组和ASA组,每组按再灌注时间分为6h、24h、3d、5d和7d,每个亚组6只大鼠。采用改良Zea Longa线栓法制作大脑中动脉阻塞(2h)模型。ASA组于再灌注后即刻及每日清晨腹腔注射ASA(80mg/kg,溶解于1.5ml的10%L-赖氨酸等渗盐水溶液中)1次。对照组在相同时间点注射10%L-赖氨酸1.5ml。采用免疫组织化学方法检测脑组织VEGF和Survivin的表达。结果 CI/RP后,VEGF和Survivin主要在梗死侧缺血中心区及周边区表达,神经元和血管内皮细胞均有表达。VEGF和Survivin的表达趋势是一致的。在梗死中心区,CI/RP后6h即呈强阳性表达,细胞数最多,随后细胞数逐渐减少,7d最少;而在梗死周边区,两者的表达是先逐渐增加,随后逐渐减少,7d最少。ASA能够进一步增加缺血诱导的VEGF和Survivin的表达。结论缺血可诱导大鼠脑梗死侧半球神经元、部分血管内皮细胞内VEGF、Survivin的表达,ASA增加缺血脑组织VEGF和Survivin的表达,可能是其神经保护机制之一。
Objective To observe the expressions of vascular endothelial growth factor（VEGF）and survivin after focal cerebral ischemia/reperfusion（CI/RP）in rats and the effect of aspirin on their expressions.Methods A total of 60 healthy male Sprague-Dawley rats were randomly assigned to the control group and aspirin group（n=30 in each group）.Each group was further divided into five subgroups according to the reperfusion time of 6h,24h,3d,5d and 7d after vessel occlusion（n=6 in each subgroup）.A model of temporary middle cerebral artery occlusion（2h）was established by suture method.Aspirin（80mg/kg dissolved in 1.5ml 10% L-Lysine normal saline solution）was intraperitoneally injected immediately after reperfusion and once every early morning in the aspirin groups;And 1.5ml 10% L-Lysine was injected intraperitoneally in the control group at the same time points as the aspirin groups.Immunohistochemical methods were used to detect VEGF and Survivin expression of ischemic brain tissues.Results VEGF and Survivin was mainly expressed in the infarct core and the peri-infarct areas of ischemic side.Both neurons and endothelial cells could be expressed them.Expressions of Survivin and VEGF in haved consistency.In the infarct core,the numbers of VEGF-and Survivin-expressing cells were maximum at 6 hours after reperfusion,then decreased gradually,and reached to minimum at 7day 7.In peri-infarct areas,the numbers of VEGF-and Survivin-expressing cells were gradually increased and then reduced,and reached to minimum at on 7day 7.Aspirin could be able to increase the expression of VEGF and Survivin after CI/RP.Conclusion Conclusion Cerebral ischemia may induce the expressions of VEGF and Survivin in neurons and endothelial cells of rats.And the up-regulation of VEGF and Survivin expressions after ischemia may be one of neuroprotective mechanisms of aspirin.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2012年第9期819-823,共5页
Journal of Apoplexy and Nervous Diseases
基金
辽宁省科技攻关项目计划资助课题(2004225003-16)
关键词
脑缺血
血管内皮生长因子
存活素
阿司匹林
Brain ischemia
Vascular endothelial growth factor
Survivin
Aspirin
