摘要
以原代培养的SD新生乳鼠心肌细胞为研究对象,建立缺氧/复氧(A/R)损伤和缺氧预适应(APC)保护模型。通过RT-PCR法和Western blot法检测阴离子交换蛋白(AEs)各亚型AE1、AE2和AE3在A/R损伤与APC保护中表达是否存在差异。结果发现,A/R损伤后AE2表达增加,APC可抑制其增加;但AE1和AE3经APC处理后,表达却明显上调;提示:AE2可能参与心肌细胞A/R损伤,AE1和AE3则可能参与了心肌细胞APC的保护作用。
It explored the differential expression of AEs proteins in the anoxia/reoxygenation（A/R） injury and anoxia preconditioning（APC） of cardiomyocytes.RT-PCR and Western blotting were used to measure the different AE isoform（AE1,AE2,AE3） mRNA and protein expression in primary neonatal cardiomyocytes A/R model and APC model respectively.The results showed that AE2 protein was upregulated after A/R injury,which can be suppressed by APC.However,APC induced the high level expression of AE1 and AE3 protein.It suggested that AE2 protein may be participate in the A/R injury and AE1,AE3 may be involved in the process of the protection induced by APC.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2011年第6期568-573,共6页
Journal of Nanchang University(Natural Science)
基金
国家自然科学基金资助项目(30760075
30960449)
关键词
阴离子交换蛋白
氯离子
缺氧复氧
缺氧预适应
心肌细胞
anion exchangers
chloride
anoxia/reoxygenation
anoxia preconditioning
cardiomyocyte
