摘要
目的:建立毛冬青有效部位中总黄酮含量的测定方法。方法:采用紫外分光光度法,以硝酸铝、亚硝酸钠及氢氧化钠为显色剂,以芦丁为对照品,在510 nm波长处对毛冬青有效部位中总黄酮进行含量测定。结果:芦丁在0.99-49.50 mg/L范围内呈良好的线性关系,r=0.999 1,平均回收率为98.9%,RSD为1.52%(n=6)。结论:所建立的方法准确、简便、快速,重现性好,适用于毛冬青有效部位中总黄酮含量测定。
Objective：To establish the method for the content determination of total flavonoids in the effective fraction of Ilex pubescens Hook.Methods：Ultraviolet spectrophotometry was applied using aluminum nitrat、sodium nitrite and NaOH as colour-display reagent,rutin as reference substance.The detection wavelength was set at 510 nm.Results：The linear range of rutin was 0.99-49.5 mg/L（r=0.9991）with an average recovery of 98.9%,RSD=1.52%（n=6）.Conclusion：The method was accuracy,simple and rapid,the reproducibility was good,so the method was suitable for the content determination of total flavonoids in the effective fraction of Ilex pubescens Hook.
出处
《中医学报》
CAS
2011年第7期826-827,共2页
Acta Chinese Medicine
基金
国家"重大新药"创制科技重大专项(编号:2009ZX09103-324)
关键词
毛冬青
总黄酮
紫外分光光度法
含量测定
the effective fraction of Ilex pubescens hook
total Flavonoids
ultraviolet spectrophotometry
content determination
