摘要
目的探讨sCD40L、IL-6和TNF-α在冠心病(CHD)的发生、发展中所起的作用及其之间的相互关系和临床意义,为CHD患者的早期诊断、病情监测、疗效观察和评估预后提供重要实验指标。方法收集CHD患者100例纳入实验组,实验组包括稳定型心绞痛(SA)组和不稳定型心绞痛(UA)组,每组各50例;将本院门诊体检健康者50例纳入对照组,两组间性别和年龄差异无显著性。在临床治疗前,从研究对象的肘静脉抽取空腹静脉血3mL,离心收集血清,分装保存。采用市售ELISA试剂盒进行检测清中sCD40L、IL-6和TNF-α,进行酶标仪比色,直线回归处理数据。结果实验组(包括SA和UA组)sCD40L、IL-6和TNF-α的表达明显高于对照组,差异显著,具有统计学意义(P<0.05),SA与UA组之间比较差别并不显著(P>0.05);血清中sCD40L、IL-6和TNF-α三者两两之间呈高度直线相关。结论血清sCD40L、IL-6和TNF-α水平测定可早期反应CHD的病理生理过程,对CHD的诊断具有实用价值,可作为CHD患者的早期诊断、病情监测、疗效观察和评估预后等重要指标。
Objective To investigate the sCD40L,IL-6 and TNF-α in the development of coronary heart disease（CHD） in the role of the interaction between the Relationship and clinical significance of early diagnosis in patients with coronary heart disease,disease monitoring,observation and assessment of prognosis of important experimental means Standard.Methods Inpatient CHD patients with 100 cases in the experimental group,the experimental group,including stable angina（SA） group and unstable angina（UA） group,n=50 cases;will Outpatient hospital into 50 cases of healthy volunteers control group,gender and age differences between the two groups was not significant.In clinical treatment,from the research cubital vein study object fasting blood 3mL,the serum collected by centrifugation,packaging preservation.Using commercially available ELISA kit review Clearance in measuring sCD40L,IL-6 and TNF-α,the micro-plate colorimetric,linear regression with the data.Results The experimental group（including the SA and UA group） sCD40L,IL-6 and TNF-α expression was significantly higher,the difference was statistically significant（P0.05）,SA and UA Not much difference between the comparison group（P0.05）;sCD40L,IL-6 and TNF-α three pairs were highly linear correlation.Conclusion Serum sCD40L,IL-6 and TNF-α levels may be an early reflection of the highest measured pathophysiological process of CHD,the diagnosis of CHD has practical value,as early diagnosis of patients with CHD,disease surveillance measurement,observation and assessment of prognostic indicators.
出处
《中国医药指南》
2011年第9期5-6,共2页
Guide of China Medicine
基金
广东省韶关市医药卫生科研计划项目(Y10072)
