摘要
目的分析自动生化仪中重氮试剂对尿素测定是否存在交叉污染及其产生原N并提出解决方法。方法用迈瑞BS-300全自动生化分析仪将新鲜混合血清单独进行尿素测定，然后将同份标本分别置于总胆红素（TB）或直接胆红素（DB）测试项目后再进行尿素测定，记录其结果并进行统计学分析；将重氮试剂一系列稀释作为标本来测定尿素，分析其对尿素自动化测定有无影响及影响程度。结果重氮法原理中直接胆红素试剂对尿素测定结果没有影响，总胆红素试剂对尿素会产生明显的正干扰。结论检验人员使用全自动生化仪时，不仅要熟悉仪器工作流程、试剂组成、测定原理等，更要注意试剂间的交叉污染，并根据仪器工作特点制定相应的措施来消除交叉污染，从根本上保证实验数据的可靠性，为临床提供客观真实的诊疗依据。
Objective To analyse the exist and the cause of cross-contamination in the detection of urea by automatic bio- chemical analyzer,and put forward the resolvent. Methods Separately detected the urea of the fresh mixed serum by Mairui BS-300 automatic biochemical analyzer,then re-detected them after the detection sof total bilirubin or direct biliru- bin,and recorded the result and processed the statistics analysis. Detected the urea of the Diazonium reagent by a series of dilutions,then analysed the influence and degree. Results Direct bilirubin by Diazo principle had not influence to the de- tection of urea,but total bilirubin had obvious interference. Conclusion The laboratory worker not only know well the work process, the constitution of the reagent,the detecting principle ,and also notice the cross-contamination between the reagents,then establish the relevant measure to eliminate them according to the instrument character,so ensure the relia- bilities of the experiment data basically,and offer actual and impersonal envidence for the clinical diagnosis and treat.
出处
《现代检验医学杂志》
CAS
2009年第4期35-36,共2页
Journal of Modern Laboratory Medicine
