摘要
目的探讨白细胞介素（IL）-17^＋CD4^＋炎症性T细胞（Th17细胞）和Foxp3^＋CD4^＋调节性T细胞在类风湿关节炎（RA）的相互关系及可能机制。方法采用前瞻性随机开放研究。测定RA患者外周血IL-17^＋CD4^＋T细胞及Foxp3^＋CD4^＋T细胞的比例变化及其相关细胞因子转化生长因子（TGF）-β、IL-6、IL-23和IL-17水平。结果RA患者外周血IL-17^＋CD4^＋T细胞明显升高（P〈0．01），而Foxp3^＋CD4^＋T细胞明显降低（P〈0．01）。同时，血清中IL-6、IL-23和IL-17水平明显升高（均P〈0．01），而TGF—β水平无明显变化（P〉0．05）。结论RA患者Th17细胞数量增高，而调节性T细胞数量减少，体内相关细胞因子的变化是引起上述改变的重要原因。
Objective To identify interleukin （IL）-17-producing T cells and Foxp3 positive CD4 positive T cells from patients with rheumatoid arthritis （RA）, and investigate their cytokine levels as well as their correlations. Methods Flow cytometry was used to analyze the subsets of T cells in peripheral blood from 39 RA patients and 40 healthy donors. I1-17, IL-6, IL-23 and transforming growth factor （TGF）-beta levels in sera of these subjects were tested by ELISA. Results IL-17^＋CD4^＋ T cells increased significantly and IL-17, IL-6 and IL-23 elevated markedly in peripheral blood of RA patients compared to healthy donors （P〈 0.01 ）. However,Foxp3^＋CD4^＋T cells decreased evidently （P〈0.01） and TGF-beta did not increase in RA patients when compared tO healthy donors （P〉0.05）. Conclusion This study suggests that Thl7 cells predominance coupled with relatively insufficient CD4^＋ regulatory T cells in RA is mainly caused by alterations of related cytokines.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2009年第7期439-442,共4页
Chinese Journal of Rheumatology
基金
国家自然基金青年基金（30700751）
教育部科学技术研究重点项目（106004）.志谢：感谢北京大学生命科学学院杜丽颖教授,北京大学血液病研究所刘艳荣教授、王亚哲医师、王东侠医师在本研究中提供的指导和帮助
