摘要
选用合适的引物,利用PCR快速扩增反应检测猪肉中的沙门氏菌。根据沙门氏菌的Fimy基因设计一对引物,对不同血清型的沙门氏菌和非沙门氏菌进行PCR检测,沙门氏菌均能检测出特异条带,而非沙门氏菌无一能检测出特异条带。将沙门氏菌和非沙门氏菌混合培养,对混合培养物提取DNA模板进行检测,结果呈阳性,而不含沙门氏菌的混合培养物则没有特异条带;对模板进行梯度稀释后PCR扩增检测,当DNA含量只有0.045ng/μL时仍能扩增出条带,将菌液进行梯度稀释后提取DNA模板,沙门氏菌含量在102cfu/mL时能被检测出来。
Devise an appropriate set of primers and detect Salmonella in pork by PCR amplification. A set of primers was devised according to FimY gene in Salmonella to detect Salmonella of various serovars by PCR amplification. The result showed that Salmonella can be detected while others can＇t. When Salmonella was mixed with other bacteria and incubation, it can still be detected, but other cultures which contain no Salmonella were detected negative. It was detectable when the concentration of DNA was at 0.045 ng/uL or the bacteria concentration at 102 cfu/mL.
出处
《食品研究与开发》
CAS
北大核心
2009年第5期128-131,共4页
Food Research and Development
基金
西安市科技攻关项目(YF07097)
