摘要
目的了解慢性乙型肝炎时，Ｃ－反应蛋白（ＣＲＰ）在肝细胞中的表达。方法用本室建立的ＬＢＡ法检测４０例ＨＢｓＡｇ阳性慢性肝炎的肝组织和１５例ＨＢｓＡｇ阴性的正常肝组织的ＣＲＰ原位表达；用免疫双标记染色检测肝细胞中ＣＲＰ、ＨＢｓＡｇ的表达。结果４０例肝炎标本ＣＲＰ均呈阳性，表达位置和形态与ＨＢｓＡｇ相似；免疫双标记染色显示肝细胞中ＣＲＰ与ＨＢｓＡｇ有重叠。此外，Ｔ淋巴细胞也表达ＣＲＰ。结论ＣＲＰ可与ＨＢｓＡｇ形成复合物。
Objective To detect the localization of CRP in liver tissue of HBV chronic hepatitis by the labelled biotin avidin (LBA) technique. Methods The LBA was set up in our laboratory to detect the localization of CRP in liver tissue of 40 HBsAg positive chronic hepatitis patients and 15 HBsAg negative normal individuals, as well as immunodouble labelling technique was used to detect the expression of CRP and HBsAg in hepatocytes. Results CRP positive substance appeared in all 40 HBV hepatitis samples. CRP presented a similar distribution and morphological pattern as HBsAg did in hepatocytes. The expression of CRP and HBsAg in hepatocytes was eclipsed by immunodouble labelling technique. The expression of CRP could be detected also in lymphocytes. Conclusion CRP HBsAg compound can be formed in hepatocytes. CRP maybe play a role in the pathogenic mechanism of HBV chronic hepatitis.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1998年第1期17-19,共3页
Chinese Journal of Infectious Diseases
