摘要
目的探讨WT1基因在儿童恶性造血系统疾病中表达的意义。方法采用巢式RT-PCR观察104例恶性造血系统疾病患儿WT1基因表达的相对水平;其中男69例,女35例;年龄1.5～14.0岁;同期检测72例成人恶性造血系统疾病患者作比较,选择12例健康体检者及10例非恶性造血系统疾病患儿骨髓标本或外周血作阴性对照(男9例,女13例;年龄3～12岁)。结果68例急性白血病(AL)患儿中WT1阳性表达49例(72.1%),3例慢性粒细胞白血病(CML)表达阴性,23例恶性淋巴瘤中7例阳性(30.4%),10例骨髓增生异常综合征(MDS)中阳性3例(30.0%),AL与MDS、CML及恶性淋巴瘤WT1阳性表达率比较差异均有显著性意义(Pa<0.01);而在健康体检者及造血系统非恶性造血系统疾病患儿中均表达阴性。各组与成人患者比较无年龄上差异(Pa>0.05),WT1基因表达在ALL均阴性,与急性粒细胞白血病(ALL)比较无显著性差异,在MDS中难治性贫血组显著低于原始细胞增多性难治性贫血及转化型原始细胞增多性难治性贫血(Pa<0.05)。结论WT1基因在恶性造血系统疾病患者中高表达,可作为检测AL微小残留及预测复发的肿瘤标志物;其与MDS疾病进程关系密切,并可作为对MDS疾病发展的高危评估指标之一。
Objective To explore the expression of Wilms＇ tumor gene （ WT1 ） in children with of malignant disease of hematopietic system. Methods Relative levels of WT1 mRNA expression in 104 children patients with various maligant disease of hematopietic system （69 male and 35 female; age ranging 1.5 -14.0 years old） were examined by nested reverse transcriptase -polymerase chain reaction （RTPCR）. Seventy - two adult patients also were examined. Samples of 12 healthy children and 10 children of no maligant disease of hematopietic system were used as normal control group （9 male and 3 female, age ranging 3.0 - 12.0 years old）. Results WTt mRNA positive expression ratio was 72.1% （49/68）in patients with AL, the positive expression ratio had no significant difference in children compared with duh. Three patients with chronic myeloid leukemia（CML） were examined negative expression. The positive expression ratio was 30.4% （7/23） in patients with malignant lymphoma while 30.0% （ 3/10 ） in patients with myelodysplastic syndrome （ MDS ）. Negative expression were examined in healthy children and children of no hematopicetic mailgnancies. The positive expression ratio had significant difference in AL compared with MDS and malignant lymphoma;It was higher in refractory anemia with excess of blast （RAEB） and transformed RAEB group than RA group. Conclusions The abnormal overexpression of WT1 gene is observed in majority of children with maligant disease of hematopietic system. It can be used as a tumor marker of AL, to monitoring minimal residual disease and predicting relapse. The expression of WT1 might be associated with the development of MDS. and it can be used for risk assessment.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2008年第21期1678-1679,共2页
Journal of Applied Clinical Pediatrics
关键词
WT1基因
造血系统
肿瘤
巢式反转录-聚合酶链反应
儿童
WT1 gene
hematopietic system
tumor
nested reverse transcriptase - ploymerase chain reaction
child
