摘要
目的:采用液相色谱法同时测定天山雪莲中紫丁香苷、绿原酸和芦丁的含量。方法:Kromasil ODS-1色谱柱(250 mm×4.6 mm,5μm),柱温为35℃;以乙腈(A)-0.4%磷酸(B)为流动相,梯度洗脱(0 min,13%A;15 min,13%A;16 min,15%A;40 min11,15%A),流速1 mL·min^(-1);双波长检测:257 nm(检测紫丁香苷、芦丁),327 nm(检测绿原酸)。结果:紫丁香苷存5.104～51.04 μg·mL^(-1)(r=0.9998)、绿原酸在38.82～258.8μg·mL^(-1)(r=0.9991)、芦丁在39.42～262.8μg·mL^(-1)(r=0.9997)范围内都呈良好线性关系;紫丁香苷、绿原酸和芦丁的回收率分别为99.7%(RSD=1.0%),102.9%(RSD=1.5%),101.7%(RSD=1.2%);供试品溶液在12 h 内稳定。结论:该方法快速简单,线性关系良好。
Objective：To describe a method for determining syringin, chlorogenic acid and rutin in Saussurea involucrate by HPLC. Method：The determination was carried out over a Kromasil ODS - 1 column（250 mm × 4. 6 mm, 5 μm）at the temperature （35 ℃ ）. The mobile phase consisted of acetonitrile（A） -0. 4% phosphoric acid aqueous solution （B） with the gradient elution （0 min, 13 % A ; 15 min, 13 % A ; 16 min, 15 % A ;40 min, 15 % A）, the flow rate：1 mL· min^-1,and detection wavelength was set at 257 nm （detecting syringin and rutin）and 327 nm （detecting chlorogenic acid）. Results：The linear ranges were 5. 104 -51.04 μg·mL^-1 for syringin（r =0. 9998） ,38.82 - 258.8μg·mL^-1 for chlorogenic acid （ r = 0. 9991 ） and 39.42 - 262.8 μg·mL^-1 for rutin （ r = 0. 9997 ） ; The average recoveries of syringin,chlorogenic acid ,and turin were 99.7% （RSD = 1.0% ）, 102.9% （RSD = 1.5% ）, and 101.7% （ RSD = 1.2% ） ,respectively;The sample solution was steady within 12 h. Conclusion：The method is simple, and has good linear relationship.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第5期762-765,共4页
Chinese Journal of Pharmaceutical Analysis
基金
新疆维吾尔自治区重点实验室开放课题(xjys0207-2006-02)
