摘要
目的探讨胰岛素样生长因子1(IGF1)及其受体(IGF1R)在卵巢癌HO8910PM细胞增殖中的作用。方法以不同浓度的IGF1作用于HO8910PM细胞24、48、72和96 h,通过CCK-8法检测细胞增殖活性;在脂质体介导下转染特异性IGF1R小干扰RNA(siRNA),通过实时PCR和Western blotting验证其在IGF1R mRNA和蛋白表达水平的抑制效果;于转染后48 h给予IGF1刺激,通过CCK-8法测定细胞增殖活性。结果IGF1作用48 h后可明显刺激细胞的增殖(P<0.05);转染IGF1R siRNA后48 h,IGF1R mRNA和蛋白表达均明显下调(P<0.05);转染IGF1R siRNA后48 h给予IGF1刺激,IGF1的促细胞增殖效应明显被抑制(P<0.05)。结论IGF1通过IGF1R途径促进HO8910PM细胞的增殖,IGF1R siRNA可以有效抑制该作用。
Objective To investigate the effects of insulin-like growth factor 1 （IGF1） and IGF1 receptor（IGF1R） on proliferation of HO8910PM cells of ovarian cancer. Methods The effects of IGF1 of different concentration on HO8910PM cells were observed by CCK-8 assay. Transfection of IGF1R siRNA by lipofectamine 2000 to silence IGF1R gene expression in HO8910PM, the silence effects were evaluated by real-time PCR and Western blotting. Forty-eight hours after transfection, IGF1 was added on HO8910PM cells to observe the effects on proliferation by CCK-8 assay. Results Incubation with IGF1 for 48 h significantly stimulated the proliferation response of HO8910PM cells （P 〈 0.05）. IGF1R mRNA and protein expression levels were significantly decreased by transfecting IGF1R siRNA for 48 h （ P 〈 0.05）. After transfection of IGF1R siRNA for 48 h and incubation with IGF1, the stimulation of proliferation response was significantly down-regulated （P 〈 0.05）. Conclusion IGF1 stimulates the proliferation response of HO8910PM cells by IGF1R pathway. IGF1R siRNA may effectively down-regulate the effects.
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2008年第3期270-272,共3页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金(30471808)
上海市科委基金(04JC14045)~~
关键词
胰岛素样生长因子
受体
卵巢癌
小干扰RNA
增殖
insulin-like growth factor
recepter
ovarian cancer
small interference RNA
proliferation
