摘要
目的探讨肿瘤坏死因子相关凋亡诱导配体及其死亡受体5与急性冠状动脉综合征之间的关系。方法将83例研究对象分成3组,其中急性冠状动脉综合征(简称综合征)组40例,稳定型心绞痛(简称心绞痛)组21例,对照组22例。应用酶联免疫吸附法测定所有病例的血浆肿瘤坏死因子相关的凋亡诱导配体和死亡受体5水平。收集升主动脉标本22例,其中综合征组患者8例,心绞痛7例,对照组7例,应用逆转录聚合酶链反应测定其肿瘤坏死因子相关的凋亡诱导配体及其死亡受体5mRNA表达情况。结果综合征组血浆肿瘤坏死因子相关的凋亡诱导配体水平显著性升高(P<0.001)。血浆死亡受体5水平综合征组明显高于对照组(P<0.05)。在综合征组中,3支血管病变亚组的血浆肿瘤坏死因子相关凋亡诱导配体和死亡受体5水平显著高于单支血管病变亚组(P值均<0.05),其中肿瘤坏死因子相关凋亡诱导配体血浆水平同时明显高于双支血管病变亚组(P<0.05)。在双支血管病变亚组中,死亡受体5血浆水平显著高于单支血管病变亚组(P<0.05)。多元Logistic逐步回归分析显示肿瘤坏死因子相关凋亡诱导配体的风险比值为1.012(P=0.002)。综合征组升主动脉中肿瘤坏死因子相关凋亡诱导配体mRNA的表达明显增强(P<0.05)。结论升高的血浆肿瘤坏死因子相关凋亡诱导配体和死亡受体5水平与急性冠状动脉综合征的发生和严重程度密切相关。
Aim To explore the relationship between the levels of tumor necrosis factor related apoptosis inducing ligand（TRAIL） and death receptor 5（DR5） and acute coronary syndrome（ACS）.Methods The 62 patients with coronary artery disease were divided into two groups：40 in the ACS group and 21 in the stable angina pectoris（SAP） group.Twenty-two healthy volunteers served as normal coronary artery（NCA） group.Plasma TRAIL and DR5 levels from these people were assayed by enzyme-linked immunosorbent assay.Reverse transcription polymerase chain reaction was used for the detection of TRAIL and DR5 mRNA of aseencling aortas from 22 subjects, of which 8 were from the ACS group, 7 from the SAP group and 7 from the NCA group, Results Plasma TRAIL levels were significantly increased in the ACS group （ P 〈 0.001）, Patients with ACS had a markedly greater plasma DR5 level than those with NCA （ P 〈 0.05）, Plasma levels of both TRAIL and DR5 in patients with 3-vessel involved ACS were significantly higher than those in patients with 1-vessel （ P 〈 0.05）. The patients with 3-vessel involved A CS had markedly raised plasma TRAIL levels when compared with the patients with 2-vessel （ P 〈 0.05）, In the 2-vessel group, only plasma DR.5 levels had a statistical significance greater than those in the 1-vessel group （P 〈 0.05）. Logistic stepwise regression analysis revealed that odds ratio of TRAIL is 1.012 （ P = 0.002）. Ascending aortas from the ACS group exhibited markedly higher TRAIL expression as compared with those from the SAP and NCA group（ P 〈 0.05 ）. Conclusions Increased plasma TRAIL and DR5 levels are closely associated with the presence and severity of ACS.
出处
《中国动脉硬化杂志》
CAS
CSCD
2007年第11期842-846,共5页
Chinese Journal of Arteriosclerosis
