摘要
以高含油量的甘蓝型油菜品系1481、1489以及1481×206的F1为供体材料,对影响游离小孢子胚胎发生的部分因素——供体植株基因型、小孢子培养密度、小孢子培养的合适时期进行了研究,同时采用近红外反射光谱分析技术对高含油量甘蓝型油菜品系1481和1489获得的DH系种子含油量进行了测定。研究结果表明:材料1481的小孢子产胚量较高,达34.9个/蕾;三种材料的小孢子培养合适密度均为3蕾/皿;大田种植的甘蓝型油菜产胚量以初花至初花后1周左右的时间最高;所获得的40个DH系含油量中有21个DH系的含油量与对照中双4号的含油量之间存在显著差异,有13个DH系的含油量比供体亲本高,其中WD-33、WD-34两个DH系的含油量高达47%以上,可以作为选育或转育高含油量新品种的材料。
The effects of genotype,cultural density and inflorescence stage on embryo production from microspores of three high oil content lines were investigated. The results showed that there were significant genotypic differences for embryogenesis among these genotypes. But the appropriate density of microspore culture of these three materials was 3 buds/plate. The highest embryogenesis frequency was obtained from buds sampled one week after initial efflorescence. Analysis of oil content by the NIRS （near-infrared reflectance spectroscopy） showed that microspore culture was an effective approach for selecting high oil content DH lines. Among 40 DH lines derived from 1481 and 1489,oil content of two DH lines,WD-33 and WD-34,reach above 47%.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2007年第4期382-386,共5页
Chinese Journal of Oil Crop Sciences
基金
国家科技支撑计划(2006BAD01A04)
国家863计划项目(2006AA10Z1B8)
关键词
甘蓝型油菜
小孢子培养
含油量
近红外光谱
Brassica napus L.
Microspore culture
Oil content
DH line
NIRS （near-infrared reflectance spectroscopy）
