摘要
以当地育成的小麦抗白粉病材料，对国外筛选到的与白粉病抗性基因Ｐｍ２及Ｐｍ４ａ紧密连锁的ＲＦＬＰ标记进行了分析。结果表明，这些ＲＦＬＰ标记即使在遗传背景不同的情况下仍可用于对Ｐｍ２及Ｐｍ４ａ基因的检测及鉴定。研究还表明，利用分子标记辅助小麦抗白粉病育种不仅可行，而且还可对育成的抗病品系所携具体抗性基因进行精确鉴定。
RFLP markers tightly linked to P m2 and P m4a genes were tested on locally developed powdery mildew resistant wheat strains. The results showed that the linked markers screened from near isogenic lines (NILS) of common wheat cultivar Chancellor were proved to be also applicable under different genetic backgrounds. It was demonstrated that molecular markers not only facilitated the breeding process, but also precisely identified the specific genes incorporated in newly bred resistant strains. Marker assisting technique might alleviate significantly the tedious procedure in generally required inoculation and identification using different cultures or isolates.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1997年第2期1-5,共5页
Journal of Nanjing Agricultural University
基金
国家自然科学基金
关键词
小麦
白粉病
抗病育种
近等基因系
分子标记
wheat
wheat powdery mildew
disease resistant breeding
near isogenic lines
RFLP marker
