摘要
在高精料日粮日粮条件下（6：4），利用体外静态模拟发酵法，设计4个水平3个重复，通过分别添加0mg／L、50mg／L、100mg／L、150mg／L海南霉素于发酵瓶中，研究海南霉素对反刍动物瘤胃发酵调控的影响。结果表明经恒温培养箱39℃培养72h，在设定的时间点测定pH值、产气量、氨氮含量、挥发性脂肪酸、中性洗涤纤维，发现在体外条件下海南霉素各处理组在0～24h内能减缓pH的下降，和对照组比差异都显著，但24h后却影响不大；产气方面，各时间点的海南霉素各处理组的产气量都极显著低于对照组。在72h时间点上，50mg／L、100mg／L、150mg／L海南霉素组分别比对照纽减少产气25．1％、28．2％、20．9％，和对照组都差异极显著（P〈0．01）；海南霉素能减少瘤胃液中氨氮含量，72h后，50mg／L、100mg／L与150mg／L处理组比对照组氨氮浓度分别减少31．9％、33．4％、28．7％，差异极显著（p〈0．01）；同时可以改善瘤胃发酵类型，海南霉素各处理组能降低乙酸丙酸比例，差异显著（P〈0．05），而72h后各处理组能提高总挥发性脂肪酸的生成量，但影响不大（P〉0．05）；对中性洗涤纤维NDF影响不大。
In this experiment, the effects of Hainanmycin on artifical rumen fermentation in the high concentrate diet was studied in vitro. 4 defferrent feeding levels of Hainanmycin with 3 repetitions were designed, which is 0 mg/L, 50mg/L, 100mg/L and 150mg/L. During 72h foster at 39℃, the results showed that the pH values in the fermentation liquids of the four level were no significant difference （ P 〉 0.05 ） after 72h fermentation, but between 0-24h Hainanmycin levels could slow down the drop of PH; Hainanmycin levels could reduce volume of gas production, the 50mg/L. 100mg/L and 150mg/Llevels could reduce 25.1%.28.2%.20.9% each（ P 〈 0.01 ）. 50mg/L.100mg/L and 150mg/L defferrent levels of Hainanmycin could also reduce NH3--N concertation 31.9% ,33. 4% .28. 7% comparing control （ P 〈 0.01 ） ; And the radio of acetate to propionate （A/P） was decreased significantly by using Hainanmycin （ P 〈 0.05 ）, and at 72h, Hainanmycin each level could increase the amount of total VFA a little （ P〉 0.05 ）; But Hainanmycin did no much influence on NDF.
出处
《家畜生态学报》
2007年第1期41-46,共6页
Journal of Domestic Animal Ecology
基金
农业部农业结构调整重大技术研究专项(2003-09-0113)
关键词
海南霉素
人工瘤胃
体外发酵
Hainanmycin
artifical rumen fermentation
in vitro
