摘要
利用硫酸二乙酯和紫外线对嗜麦芽黄单胞菌(Xanthomonas maltophilia)复合诱变,初筛和复筛后,得到了转化率高且遗传性能稳定的α-熊果苷生产菌株BT-112。该菌株在以蔗糖为碳源、发酵液初始pH为7.5,温度为28℃,摇床转速为150 r/min及发酵时间为48 h的条件下,对苯二酚转化为α-熊果苷的转化率可达93.2%,比出发菌株转化率(73.0%)提高了27.7%。纯化产物经NMR鉴定,与标准品对比,确定为α-熊果苷。
A Xanthomonas maltophilia strain was mutated using diethyl sulfate （DES） and UV irradiation. The BT-112 strain was found to produce α-arbutin with high conversion efficiency and could be recycled without loss of activity. The optimum fermentation conditions were found to be sucrose as the carbon source, initial pH 7.5, fermentation temperature 28 ℃, shaker speed 150 r/min, and fermentation time 48 h. Under these conditions the conversion of hydroquinone to a-arbutin reached 93.2 %, which was an increase of 27.7 % over that obtained with the starting strain （70.3 % ）. The ^13C-NMR and ^1H-NMR spectra of the purified product were identical to those of an authentic sample of α-arbutin.
出处
《北京化工大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第4期13-16,共4页
Journal of Beijing University of Chemical Technology(Natural Science Edition)
关键词
α-熊果苷
黄单胞菌
发酵
诱变
α-arbutin
Xanthomonas maltophilia
fermentation
mutation
